Gao Feng, Zhao Yanjing, Zhang Yunli, Gu Yanlong, Pei Chao, Li Chen, Kong Xianghui
Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan Province, PR China.
Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan Province, PR China.
Fish Shellfish Immunol. 2024 Sep;152:109783. doi: 10.1016/j.fsi.2024.109783. Epub 2024 Jul 19.
Prostaglandin-endoperoxide synthase 2 (PTGS2), a common biological macromolecule, is pivotal for innate immunity and pathogen recognition. In this study, we identified and characterized a CcPTGS2a-like gene in the common carp (Cyprinus carpio) with an open reading frame (ORF) of 1821 bp and epidermal growth factor and peroxidase domains. Our multiple sequence analysis revealed high homology between the amino acid sequence of CcPTGS2a-like and those of its homologs in other fish. CcPTGS2a-like mRNA and protein expressions were significantly upregulated in the spleen, head kidney, liver, and gill tissues upon exposure to Aeromonas hydrophila stimulation. CcPTGS2a-like protein recognized the conserved bacterial surface components and exhibited detectable bacterial binding activity. CcPTGS2a-like overexpression before exposure to A. hydrophila notably enhanced the survival rate of common carp, concomitant with decreased bacterial burden. The NF-κB/ERK signaling pathway initiated the immune response in common carp upon infection with A. hydrophila. CcPTGS2a-like overexpression or interference in the head kidney and Epithelioma papulosum cyprinid cells could modulate the p-NF-κB (p-p-65), p-IκBα, and p-ERK1/2 levels as well as the IL-1β and IL-6 mRNA expression. These results indicated potential CcPTGS2a-like involvement in the immune response of the common carp to bacterial infections through the NF-κB/ERK signaling pathway.
前列腺素内过氧化物合酶2（PTGS2）是一种常见的生物大分子，对先天免疫和病原体识别至关重要。在本研究中，我们在鲤鱼（Cyprinus carpio）中鉴定并表征了一个CcPTGS2a样基因，其开放阅读框（ORF）为1821 bp，含有表皮生长因子和过氧化物酶结构域。我们的多序列分析显示，CcPTGS2a样的氨基酸序列与其在其他鱼类中的同源物具有高度同源性。在暴露于嗜水气单胞菌刺激后，CcPTGS2a样mRNA和蛋白表达在脾脏、头肾、肝脏和鳃组织中显著上调。CcPTGS2a样蛋白识别保守的细菌表面成分，并表现出可检测的细菌结合活性。在暴露于嗜水气单胞菌之前过表达CcPTGS2a样显著提高了鲤鱼的存活率，同时细菌载量降低。NF-κB/ERK信号通路在鲤鱼感染嗜水气单胞菌后启动免疫反应。在头肾和鲤上皮瘤细胞中过表达或干扰CcPTGS2a样可以调节p-NF-κB（p-p-65）、p-IκBα和p-ERK1/2水平以及IL-1β和IL-6 mRNA表达。这些结果表明，CcPTGS2a样可能通过NF-κB/ERK信号通路参与鲤鱼对细菌感染的免疫反应。
