College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Chengdu Customs Technology Center, Chengdu, 610041, China.
Biosens Bioelectron. 2024 Nov 1;263:116577. doi: 10.1016/j.bios.2024.116577. Epub 2024 Jul 20.
Implementation of cost-effective, reliable, and efficient technologies for the sensitive, rapid, and accurate detection of pesticide residues in agriproducts presents a promising solution to the escalating food safety concerns. Herein, a high-performance surface-enhanced Raman scattering (SERS) aptasensor based on nanotag (AuNS@4-MBN@Ag-aptamer) was introduced for ultrasensitive, reliable, and interference-free detection of chlorpyrifos (CPF). This aptasensor featured star-shaped bimetallic nanotag as the principal Raman signal enhancement material and 4-mercaptobenzonitrile (4-MBN) as "biological-silent"-window reporter (at 2228 cm). Moreover, cDNA-linked FeO@AuNPs (FA-cDNA) served as magnetic substrates to simplify the separation process of FA-cDNA-combined nanotags. In the aptasensor, the formation of FA-cDNA-aptamer-AuNS@4-MBN@Ag hybrids was hindered by CPF, and its Raman intensity decreased with increasing CPF concentration. Under optimal SERS conditions, the aptasensor exhibited a broad linear detection range from 2.5 × 10 to 5.0 × 10 pg⋅mL, with an impressively low limit of detection of 220.35 pg⋅mL (signal-to-noise ratio = 3). The selectivity and reproducibility assessments highlighted its exceptional sensitivity and interference-free capabilities. Furthermore, practical applications on wheat and apples demonstrated satisfactory spiked recovery rates, ranging from 89.61% to 107.33% (relative standard deviation ≤ 14.55%). Consequently, the high-performance "biological-silent"-window nanotag-based aptasensor is a promising tool for monitoring trace CPF in complex matrices.
为解决日益严峻的食品安全问题，实现农产食品中农药残留的敏感、快速、准确检测，经济高效、可靠实用、环境友好的技术手段的应用前景十分广阔。本文构建了一种基于纳米标签(AuNS@4-MBN@Ag-适配体)的高性能表面增强拉曼散射(SERS)适配体传感器，用于超灵敏、可靠、无干扰地检测氯吡硫磷(CPF)。该传感器以星型双金属纳米标签为主要拉曼信号增强材料，4-巯基苯并腈(4-MBN)为“生物静默”-窗口报告分子(在 2228cm 处)。此外，cDNA 连接的 FeO@AuNPs(FA-cDNA)作为磁性基质，简化了 FA-cDNA 结合纳米标签的分离过程。在适配体传感器中，CPF 的存在阻碍了 FA-cDNA-适配体-AuNS@4-MBN@Ag 杂交体的形成，其拉曼强度随 CPF 浓度的增加而降低。在最佳 SERS 条件下，该适配体传感器的线性检测范围为 2.5×10 到 5.0×10 pg⋅mL，检测限低至 220.35 pg⋅mL(信噪比 = 3)。选择性和重现性评估突出了其卓越的灵敏度和无干扰能力。此外，在小麦和苹果中的实际应用表明，该方法具有令人满意的加标回收率，范围为 89.61%到 107.33%(相对标准偏差 ≤ 14.55%)。因此，基于高性能“生物静默”-窗口纳米标签的适配体传感器是一种用于监测复杂基质中痕量 CPF 的有前途的工具。
