Laboratory of Environmental Toxicology, Department of Sanitary and Environmental Engineering, Federal University of Santa Catarina, Florianópolis, SC 88040-970, Brazil.
Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN, 55108, USA.
Mar Pollut Bull. 2024 Sep;206:116696. doi: 10.1016/j.marpolbul.2024.116696. Epub 2024 Jul 22.
The activities of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione-S-transferase (GST) were evaluated in the gills (GI) and digestive gland (DG) of Magallana gigas oysters exposed to tamoxifen (TAM) at environmental concentrations of 10 and 100 ng L for 1 and 4 days. A higher CAT activity in the GI and DG and higher GPx activity only in the DG was observed of oysters exposed to both concentrations after 1 day. Furthermore, a significant increase in GR and G6PDH, was detected in the DG after 1 day of exposure to 10 ng L and only G6PDH activity increase after 1 day of exposure to 10 ng L in the GI. This suggests that the DG is a tissue more sensitive to TAM exposure and was confirmed with the individual Integrated Biomarker Response version 2 index (IBRv2i), highlighting the acute stress caused by TAM and a cellular adaptation.
研究了暴露于环境浓度为 10 和 100ng/L 的他莫昔芬（TAM）1 和 4 天后，美洲大赤贝（Magallana gigas）鳃（GI）和消化腺（DG）中的过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、葡萄糖-6-磷酸脱氢酶（G6PDH）和谷胱甘肽-S-转移酶（GST）的活性。结果表明，1 天后，暴露于两种浓度下的贝类 GI 和 DG 中的 CAT 活性更高，DG 中的 GPx 活性更高。此外，暴露于 10ng/L 时，DG 中的 GR 和 G6PDH 活性显著增加，而仅在 GI 中暴露于 10ng/L 时，G6PDH 活性增加。这表明 DG 是对 TAM 暴露更敏感的组织，并通过个体综合生物标志物反应第 2 版指数（IBRv2i）得到了证实，突出了 TAM 引起的急性应激和细胞适应。
