Department of Intensive Care Unit, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Wenzhou Medical University, Wenzhou, China.
Int J Hyperthermia. 2024;41(1):2382162. doi: 10.1080/02656736.2024.2382162. Epub 2024 Jul 23.
Central nervous system (CNS) injury is the most prominent feature of heatstroke and the hippocampus is prone to damage. However, the mechanisms underlying the heatstroke-induced hippocampal injury remain unclear. Hyperbaric oxygen (HBO) therapy prevents CNS injury in heatstroke mice. However, the underlying mechanisms of HBO in heatstroke-induced hippocampal injury remain unclear. This study aimed to elucidate the protective effects of HBO against hippocampal injury and its potential role in microglial pyroptosis in heatstroke rats. A rat heatstroke model and a heat stress model with a mouse microglial cell line (BV2) were, respectively, used to illustrate the effect of HBO on heat-induced microglial pyroptosis and . We used a combination of molecular and histological methods to assess microglial pyroptosis and neuroinflammation both and . The results revealed that HBO improved heatstroke-induced survival outcomes, hippocampal injury, and neurological dysfunction in rats. In addition, HBO mitigates microglial pyroptosis and reduces the expression of pro-inflammatory cytokines in the hippocampus of heatstroke rats. experiments showed that HBO attenuated BV2 cell injury under heat stress. Furthermore, HBO prevented heat-induced pyroptosis of BV2 cells, and the expression of pro-inflammatory cytokines IL-18 and IL-1β was reduced. Mechanistically, HBO alleviates heatstroke-induced neuroinflammation and hippocampal injury by preventing microglial pyroptosis. In conclusion, HBO attenuates heatstroke-induced neuroinflammation and hippocampal injury by inhibiting microglial pyroptosis.
中枢神经系统（CNS）损伤是中暑的最突出特征，海马体容易受损。然而，中暑引起的海马体损伤的机制尚不清楚。高压氧（HBO）治疗可预防中暑小鼠的中枢神经系统损伤。然而，HBO 在中暑引起的海马体损伤中的作用机制尚不清楚。本研究旨在阐明 HBO 对海马体损伤的保护作用及其在中暑大鼠小胶质细胞焦亡中的潜在作用。分别使用大鼠中暑模型和小鼠小胶质细胞系（BV2）的热应激模型来阐明 HBO 对热诱导的小胶质细胞焦亡的影响。我们使用分子和组织学方法相结合来评估热诱导的小胶质细胞焦亡和神经炎症。结果表明，HBO 改善了中暑引起的大鼠生存结果、海马体损伤和神经功能障碍。此外，HBO 减轻了中暑大鼠海马体中小胶质细胞焦亡和促炎细胞因子的表达。体外实验表明，HBO 减轻了热应激下 BV2 细胞的损伤。此外，HBO 可防止 BV2 细胞的热诱导性细胞焦亡，减少促炎细胞因子 IL-18 和 IL-1β 的表达。机制上，HBO 通过抑制小胶质细胞焦亡来减轻中暑引起的神经炎症和海马体损伤。综上所述，HBO 通过抑制小胶质细胞焦亡来减轻中暑引起的神经炎症和海马体损伤。
