Division of Infectious Diseases, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Division of Internal Medicine, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Sci Rep. 2024 Jul 23;14(1):16888. doi: 10.1038/s41598-024-67009-6.
Tick-borne diseases in animals are increasing rapidly worldwide, but there is insufficient information about tick-borne diseases infecting dogs in southern Egypt. Thus, in the current study, we detected the presence of Anaplasma marginale (A. marginale) and Babesia canis vogeli (B. canis vogeli) in the blood of dogs. The results revealed that 4/100 (4%) were positive, and a higher infection rate was found in males (75%), than females (25%). The phylogenetic analysis for the major surface protein 4 (msp4) gene in this study was compared with amplicons separate from other reported isolates with alignment by identity 100% with cattle and camels from Egypt, and the phylogenetic analysis for the B. canis vogeli small subunit ribosomal RNA (SSU rRNA) gene in this study identified identity by 99.89% with dogs from Egypt. This report is considered the first report in southern Egypt about A. marginale in dogs based on the sequence analysis of the msp4 gene, providing new data for the classification and identification of A. marginale in dogs compared to A. marginale isolated from other animals in southern Egypt.
动物传播的疾病在全球范围内迅速增加，但关于埃及南部感染犬只的虫媒传染病的信息还不够充分。因此，在目前的研究中，我们检测了犬只血液中的边缘无浆体（A. marginale）和卵形巴贝斯虫（B. canis vogeli）的存在情况。结果显示，有 4/100（4%）的犬只呈阳性，雄性（75%）的感染率高于雌性（25%）。本研究中主要表面蛋白 4（msp4）基因的系统发育分析与其他报道的分离株的扩增子进行了比较，与埃及的牛和骆驼的同源性为 100%，本研究中卵形巴贝斯虫小亚基核糖体 RNA（SSU rRNA）基因的系统发育分析与埃及的犬只的同源性为 99.89%。本报告被认为是埃及南部关于犬只感染边缘无浆体的首例报道，基于 msp4 基因的序列分析，为犬只边缘无浆体的分类和鉴定提供了新的数据，与埃及南部从其他动物中分离的边缘无浆体相比。
