Institute of Virology, Technical University of Munich, School of Medicine/Helmholtz Munich, Munich, Germany.
Infectious Diseases Therapeutic Research Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon, Republic of Korea.
Methods Mol Biol. 2024;2837:207-218. doi: 10.1007/978-1-0716-4027-2_18.
Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.
通过静脉途径感染携带复制型乙型肝炎病毒基因组的重组腺相关病毒（rAAV-HBV）的小鼠在肝细胞中建立持续的乙型肝炎病毒复制，并发展为免疫耐受。它们可作为评估抗病毒免疫和评估慢性乙型肝炎病毒感染潜在治疗方法的模型。结合选择的乙型肝炎病毒变异株和不同的小鼠基因型，可以解决广泛的研究问题。本章描述了 rAAV-HBV 小鼠模型的基本原理、rAAV-HBV 的生产和纯化方法，最后介绍了体内应用。
