Kishi J, Kawai T, Hayakawa T
Arch Oral Biol. 1985;30(7):571-6. doi: 10.1016/0003-9969(85)90059-7.
The development of an antiserum, monospecific to the collagenase inhibitor, from bovine dental pulps permitted localization of immunoreactive inhibitor protein, by means of both immunofluorescence and immunoperoxidase-staining techniques in sections of bovine dental pulps. The immunoreactive inhibitor protein in bovine dental pulps is present both in cells and extracellular matrices. When cultured in Eagle minimal essential medium, coronal pulps from bovine-unerupted teeth were shown, by assay of the medium, to produce only about 1/10 of the amount of inhibitor produced by the root pulps. When compared by immunohistochemical observation, however, essentially no differences in fluorescent activity was found between coronal and root pulps. Specific cytoplasmic staining was seen both in explanted root-pulp tissues and in immature fibroblast-like pulp cells from monolayer cell cultures of bovine root pulps, which indicate that the pulp cells are responsible for inhibitor production. Sections of dental follicle and gingiva from the same animal, showed a distribution of immunoreactive inhibitor protein similar to that in dental pulps.
从牛牙髓中研制出一种对胶原酶抑制剂具有单特异性的抗血清，借助免疫荧光和免疫过氧化物酶染色技术，可在牛牙髓切片中定位免疫反应性抑制剂蛋白。牛牙髓中的免疫反应性抑制剂蛋白存在于细胞和细胞外基质中。通过对培养基的检测发现，当在伊格尔基本培养基中培养时，来自未萌出牛牙的冠髓产生的抑制剂量仅约为根髓产生量的1/10。然而，通过免疫组织化学观察比较时，冠髓和根髓之间在荧光活性上基本没有差异。在移植的根髓组织以及来自牛根髓单层细胞培养的未成熟成纤维细胞样牙髓细胞中均可见特异性胞质染色，这表明牙髓细胞负责抑制剂的产生。同一动物的牙囊和牙龈切片显示，免疫反应性抑制剂蛋白的分布与牙髓中的相似。
