State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.
Cell Rep. 2024 Aug 27;43(8):114524. doi: 10.1016/j.celrep.2024.114524. Epub 2024 Jul 23.
The transition from two-dimensional (2D) to 3D growth likely facilitated plants to colonize land, but its heterogeneity is not well understood. In this study, we utilized single-cell RNA sequencing to analyze the moss Physcomitrium patens, whose morphogenesis involves a transition from 2D to 3D growth. We profiled over 17,000 single cells covering all major vegetative tissues, including 2D filaments (chloronema and caulonema) and 3D structures (bud and gametophore). Pseudotime analyses revealed larger numbers of candidate genes that determine cell fates for 2D tip elongation or 3D bud differentiation. Using weighted gene co-expression network analysis, we identified a module that connects β-type carbonic anhydrases (βCAs) with auxin. We further validated the cellular expression patterns of βCAs and demonstrated their roles in 3D gametophore development. Overall, our study provides insights into cellular heterogeneity in a moss and identifies molecular signatures that underpin the 2D-to-3D growth transition at single-cell resolution.
从二维（2D）到三维（3D）的生长转变可能有助于植物在陆地上定殖，但它的异质性尚不清楚。在这项研究中，我们利用单细胞 RNA 测序来分析藓类植物 Physcomitrium patens，其形态发生涉及从 2D 到 3D 生长的转变。我们对超过 17000 个单细胞进行了分析，这些细胞覆盖了所有主要的营养组织，包括 2D 丝状结构（chloronema 和 caulonema）和 3D 结构（芽和配子体）。拟时分析揭示了更多决定 2D 顶端伸长或 3D 芽分化的细胞命运的候选基因。利用加权基因共表达网络分析，我们鉴定了一个与生长素相连的β型碳酸酐酶（βCAs）模块。我们进一步验证了βCAs 的细胞表达模式，并证明了它们在 3D 配子体发育中的作用。总的来说，我们的研究提供了对藓类植物细胞异质性的深入了解，并确定了在单细胞分辨率下支持 2D 到 3D 生长转变的分子特征。
