Korea Food Research Institute, Wanju 55365, Republic of Korea.
Korea Food Research Institute, Wanju 55365, Republic of Korea.
Food Chem. 2024 Nov 30;459:140281. doi: 10.1016/j.foodchem.2024.140281. Epub 2024 Jul 1.
N-Nitrosamines (NAs) pose a threat to food safety due to their carcinogenic and mutagenic properties. In this study, we developed and validated a QuEChERS-based LC-MS/MS method for the simultaneous analysis of 11 NAs in 74 processed fish meat, processed meat, and salted fish products. Sample preparation was optimized by screening two versions of QuEChERS buffer, four extraction methods, and eight purification methods. The optimal analytical approach was validated for three product categories in terms of linearity, matrix effects, accuracy, and precision. Satisfactory precision and accuracy were demonstrated, with relative recoveries of 70-120% for the 11 NAs. The limits of detection for fish meat, processed meat, and salted fish products were 0.12-7.50, 0.12-4.14, and 0.10-7.81 ng·g, respectively. Among the 11 NAs, nine were detected in all 74 samples. This methodology could be applied to monitor NA levels to ensure the safety and quality of food products.
N-亚硝胺（NAs）因其致癌和致突变特性对食品安全构成威胁。在这项研究中，我们开发并验证了一种基于 QuEChERS 的 LC-MS/MS 方法，用于同时分析 74 种加工鱼肉类、加工肉类和咸鱼制品中的 11 种 NAs。通过筛选两种版本的 QuEChERS 缓冲液、四种提取方法和八种净化方法优化了样品制备。针对三种产品类别对最佳分析方法进行了线性、基质效应、准确度和精密度验证。11 种 NAs 的相对回收率为 70-120%，表明具有良好的精密度和准确度。鱼肉、加工肉类和咸鱼制品的检出限分别为 0.12-7.50、0.12-4.14 和 0.10-7.81 ng·g。在 11 种 NAs 中，有 9 种在所有 74 个样品中均有检出。该方法可用于监测 NAs 水平，以确保食品产品的安全和质量。
