College of Food Science, Shanxi Normal University, Taiyuan, 030000, PR China.
College of Food Science, Shanxi Normal University, Taiyuan, 030000, PR China.
Plant Physiol Biochem. 2024 Sep;214:108951. doi: 10.1016/j.plaphy.2024.108951. Epub 2024 Jul 20.
Black rots lead to great economic losses in winter jujube industry. The objective of this research was to delve into the underlying mechanisms of enhanced resistance of winter jujube fruit to black rot by L-Methionine (Met) treatment. The findings revealed that the application of Met significantly curtailed lesion diameter and decay incidence in winter jujube fruit. The peroxidase (POD) activity in the Met-treated jujubes was 3.06-fold that in the control jujubes after 4 d of treatment. By day 8, the activities of phenylalanine ammonia-lyase (PAL), chitinase (CHI) and β-1,3-glucanase (GLU) in the Met-treated jujubes had surged to their zenith, being 1.39, 1.22, and 1.52 times in the control group, respectively. At the end of storage, the flavonoid and total phenol content remained 1.58 and 1.06 times than that of the control group. Based on metabolomics and transcriptomics analysis, Met treatment upregulated 6 key differentially expressed metabolites (DEMs) (succinic acid, trans-ferulic acid, salicylic acid, delphinium pigments, (S)-abscisic acid, and hesperidin-7-neohesperidin), 12 key differentially expressed genes (DEGs) (PAL, CYP73A, COMT, 4CL, CAD, POD, UGT72E, ANS, CHS, IAA, TCH4 and PR1), which were involved in phenylpropanoid biosynthesis pathway, flavonoid biosynthesis pathway and plant hormone signal transduction pathway. Further analysis revealed that the most of the enzymes, DEMs and DEGs in this study were associated with both antioxidant and disease resistance. Consequently, Met treatment enhanced disease resistance of winter jujube fruit by elevating antioxidant capacity and triggering defense response. This study might provide theoretical support for utilizing Met in the management and prevention of post-harvest black rot in winter jujube.
戊二醛处理提高冬枣果实抗采后黑腐病能力的机制研究
戊二醛处理提高冬枣果实抗采后黑腐病能力的机制研究。戊二醛处理显著抑制了冬枣果实的病斑直径和腐烂率。戊二醛处理后第 4 天，冬枣果实过氧化物酶（POD）活性是对照组的 3.06 倍。第 8 天，戊二醛处理的冬枣果实苯丙氨酸解氨酶（PAL）、几丁质酶（CHI）和β-1,3-葡聚糖酶（GLU）的活性达到峰值，分别比对照组高 1.39、1.22 和 1.52 倍。贮藏结束时，戊二醛处理的冬枣果实类黄酮和总酚含量分别比对照组高 1.58 和 1.06 倍。基于代谢组学和转录组学分析，戊二醛处理上调了 6 个关键差异表达代谢物（DEMs）（琥珀酸、反式阿魏酸、水杨酸、飞燕草色素、（S）-脱落酸、柚皮苷-新橙皮苷）和 12 个关键差异表达基因（DEGs）（PAL、CYP73A、COMT、4CL、CAD、POD、UGT72E、ANS、CHS、IAA、TCH4 和 PR1），这些基因参与了苯丙烷生物合成途径、类黄酮生物合成途径和植物激素信号转导途径。进一步分析表明，本研究中大多数酶、DEMs 和 DEGs 与抗氧化和抗病性有关。因此，戊二醛处理通过提高抗氧化能力和触发防御反应来提高冬枣果实的抗病性。本研究为利用戊二醛防治冬枣采后黑腐病提供了理论依据。
