Kleuser B, Adam G
Cell Biol Int Rep. 1985 Nov;9(11):985-92. doi: 10.1016/0309-1651(85)90064-5.
The relation between cellular rRNA content, as a measure of cell size, and the regulation of the cell cycle has been investigated for swiss 3T3 and the spontaneously transformed swiss 3T6 cell line. It is shown that the characteristic of percent of quiescent cells stimulated into the cell cycle versus cellular rRNA content is basically different for 3T3 and 3T6 cells: 3T3 cells do not enter the cell cycle below a certain threshold of cellular rRNA content, whereas 3T6 cells start proliferation without a substantial increase of rRNA. These data are interpreted as consistent with transformation of 3T6 cells being in essence their uncoupling from the requirement of normal cells of passing over a threshold of cellular rRNA content (cell size) before initiating DNA-replication.
作为细胞大小指标的细胞核糖体RNA（rRNA）含量与细胞周期调控之间的关系，已在瑞士3T3细胞及自发转化的瑞士3T6细胞系中进行了研究。结果表明，对于3T3和3T6细胞，静息细胞被刺激进入细胞周期的百分比与细胞rRNA含量之间的特征存在根本差异：3T3细胞在细胞rRNA含量低于某个阈值时不会进入细胞周期，而3T6细胞在rRNA没有大幅增加的情况下就开始增殖。这些数据被解释为与3T6细胞的转化本质上是其与正常细胞在启动DNA复制前越过细胞rRNA含量（细胞大小）阈值的要求脱钩一致。
