ALRashdi Barakat M, Massoud Diaa, Rashwan Hager K, Mahgoub Shahenda, Abuelezz Nermeen Z, Nasr Ali M, Kassab Rami B, Amin Hatem K
Department of Biology, College of Science, Jouf University, Sakaka, Saudi Arabia.
Department of Biochemistry, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, Giza, Egypt.
J Inflamm Res. 2024 Jul 16;17:4747-4763. doi: 10.2147/JIR.S463369. eCollection 2024.
Acute kidney injury (AKI) is a medical concern that is accompanied by the rapid deterioration of kidney function. It can be triggered by lipopolysaccharide (LPS) of gram-negative bacteria as it activates a complicated immune response, resulting in widespread inflammation and potential organ dysfunction. Black seed oil (BSO) is rich in beneficial constituents and has been widely used owing to its nutritional advantages.
This research is aimed to investigate the potential protective effects of BSO and its nano-formulation on AKI induced by LPS. It also aimed to compare their anti-inflammatory activity with indomethacin, a known synthetic anti-inflammatory drug.
Forty-eight mice were placed randomly into 8 groups. A single intraperitoneal (.) injection of 2.5 mg/kg B.W. of LPS was used to trigger inflammation, and pretreatment with BSO and its nano-formulation was at 0.2 mL/kg/day for 14 consecutive days. Indomethacin was used as a reference drug and its efficacy was tested alone or in combination with BSO at lower doses. Renal function was assessed using urea, creatinine, neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). Also, oxidative and inflammatory markers were assessed by measuring levels of reduced glutathione (GSH), nitric oxide (NO), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and toll-like receptor-4 (TLR-4). Histopathological examination of the kidney tissues was also performed.
The study showed that BSO and its nano-formulation had anti-inflammatory effects comparable to or better than those of indomethacin. They greatly decreased the oxidative stress and inflammatory markers induced by LPS. Their protective effect against pathological alterations in kidney tissues was significantly noticed.
BSO and its nano-formulation could be used as nephroprotective and anti-inflammatory supplements.
急性肾损伤（AKI）是一个医学关注问题，伴有肾功能的快速恶化。革兰氏阴性菌的脂多糖（LPS）可引发AKI，因为它会激活复杂的免疫反应，导致广泛炎症和潜在的器官功能障碍。黑种草籽油（BSO）富含有益成分，因其营养优势而被广泛使用。
本研究旨在探讨BSO及其纳米制剂对LPS诱导的AKI的潜在保护作用。还旨在将它们的抗炎活性与已知的合成抗炎药吲哚美辛进行比较。
48只小鼠随机分为8组。单次腹腔注射2.5mg/kg体重的LPS以引发炎症，连续14天每天以0.2mL/kg的剂量用BSO及其纳米制剂进行预处理。吲哚美辛用作参考药物，单独或与低剂量的BSO联合测试其疗效。使用尿素、肌酐、中性粒细胞明胶酶相关脂质运载蛋白（NGAL）和肾损伤分子-1（KIM-1）评估肾功能。此外，通过测量还原型谷胱甘肽（GSH）、一氧化氮（NO）、环氧化酶-2（COX-2）、肿瘤坏死因子α（TNF-α）和Toll样受体4（TLR-4）的水平来评估氧化和炎症标志物。还对肾组织进行了组织病理学检查。
研究表明，BSO及其纳米制剂具有与吲哚美辛相当或更好的抗炎作用。它们大大降低了LPS诱导的氧化应激和炎症标志物。它们对肾组织病理改变的保护作用显著。
BSO及其纳米制剂可作为肾保护和抗炎补充剂。
