Ivarsson S A, Thorell J I
Diabetes Res. 1985 Sep;2(5):249-53.
Insulin binding to erythrocytes was shown to lack any relationship to age-related factors of the erythrocyte such as the pyruvate kinase activity and the reticulocyte count in individuals with a normal erythropoiesis. After density separation of the erythrocytes in both normal adults and children and in individuals with increased erythropoiesis, a correlation to age-factors was evident (pyruvate kinase activity/insulin binding r = 0.59, p less than 0.01, reticulocyte count/insulin binding r = 0.44, p less than 0.01). An estimate of the components of variation in the binding showed that the unexplained variance is 60%. In the density separated samples the insulin binding correlated closer to the pyruvate kinase activity than to the number of reticulocytes. During treatment of haematopoietic diseases in the 4 patients with increased erythropoiesis, the disappearance rate for the reticulocyte count was much faster (T 1/2 = 9 days) than that of the insulin binding to erythrocytes (T 1/2 = 37 days) and that of pyruvate kinase activity (T 1/2 = 36 days). In one patient studied after splenectomy, the decrease in insulin binding paralleled the disappearance curve for 51Cr-tagged erythrocytes. These results suggest that insulin binds to erythrocytes, not only during the reticulocyte stage, but throughout their life span, though the binding, which is very closely related to their pyruvate kinase activity, steadily declines as the erythrocytes age.
在红细胞生成正常的个体中，胰岛素与红细胞的结合被证明与红细胞的年龄相关因素（如丙酮酸激酶活性和网织红细胞计数）没有任何关系。在正常成年人、儿童以及红细胞生成增加的个体中对红细胞进行密度分离后，与年龄因素的相关性很明显（丙酮酸激酶活性/胰岛素结合r = 0.59，p < 0.01，网织红细胞计数/胰岛素结合r = 0.44，p < 0.01）。对结合变异成分的估计表明，无法解释的变异为60%。在密度分离的样本中，胰岛素结合与丙酮酸激酶活性的相关性比与网织红细胞数量的相关性更紧密。在4例红细胞生成增加的患者治疗造血疾病期间，网织红细胞计数的消失速度（T 1/2 = 9天）比胰岛素与红细胞的结合（T 1/2 = 37天）以及丙酮酸激酶活性（T 1/2 = 36天）的消失速度快得多。在1例脾切除术后研究的患者中，胰岛素结合的下降与51Cr标记红细胞的消失曲线平行。这些结果表明，胰岛素不仅在网织红细胞阶段与红细胞结合，而且在其整个生命周期内都与红细胞结合，尽管这种结合与丙酮酸激酶活性密切相关，但随着红细胞老化，结合会稳步下降。
