Anhui Provincial Key Laboratory of Microbial Control, Anhui Provincial Key Laboratory of Forest Resources and Silviculture, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036, China.
College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
J Agric Food Chem. 2024 Aug 7;72(31):17248-17259. doi: 10.1021/acs.jafc.4c02789. Epub 2024 Jul 25.
(Hope) presents a significant threat as a dangerous wood-boring pest, inflicting substantial harm to forest trees. Investigating the olfactory sensory system of holds substantial theoretical promise for developing eco-friendly control strategies. To date, however, the olfactory perception mechanism in remains largely unknown. Therefore, we performed transcriptome sequencing of across four distinct body parts: antennae, foreleg tarsal segments, mouthparts (maxillary and labial palps), and abdomen terminals, pinpointing the odorant binding protein (OBP) genes and analyzing their expression. We found eight (5, 19, 23, 25, 29, 59, 63, 70) highly expressed in the antennae. In our competitive binding experiments, AgerOBP23 showed strong binding abilities to the pheromone component fuscumol acetate, eight plant volatiles (farnesol, -3-hexenal, nerolidol, myristol acetate, -3-hexenyl benzoate, (-)-α-cedrene, 3-ethylacetophenone, and decane), and four insecticides (chlorpyrifos, phoxim, indoxacarb, and cypermethrin). However, AgerOBP29 and AgerOBP63 did not show prominent binding activities to these tested chemicals. Through homology modeling and molecular docking, we identified the key amino acid sites involved in the binding process of AgerOBP23 to these ligands, which shed light on the molecular interactions underlying its binding specificity. Our study suggests that AgerOBP23 may serve as a potential target for future investigations of AgerOBP ligand binding. This approach is consistent with the reverse chemical ecology principle, establishing the groundwork for future studies focusing on attractant or repellent development by exploring further the molecular interactions between OBP and various compounds.
（Hope）是一种极具威胁性的蛀干害虫，对森林树木造成了严重的危害。研究 的嗅觉感受系统对于开发环保型控制策略具有重要的理论意义。然而，迄今为止， 的嗅觉感知机制在很大程度上仍不清楚。因此，我们对 的四个不同身体部位（触角、前腿跗节、口器（大颚和下唇须）和腹部末端）进行了转录组测序，鉴定了气味结合蛋白（OBP）基因，并分析了它们的表达情况。我们发现 在触角中高度表达的 8 个（5、19、23、25、29、59、63、70）。在我们的竞争结合实验中，AgerOBP23 对信息素成分 fuscumol 乙酸表现出较强的结合能力，对 8 种植物挥发物（法呢醇、-3-己烯醛、橙花叔醇、肉豆蔻酸乙酯、-3-己烯基苯甲酸酯、（-）-α-雪松烯、3-乙基苯乙酮和癸烷）和 4 种杀虫剂（毒死蜱、辛硫磷、茚虫威和氯氰菊酯）也表现出较强的结合能力。然而，AgerOBP29 和 AgerOBP63 对这些测试化学品没有表现出明显的结合活性。通过同源建模和分子对接，我们确定了 AgerOBP23 与这些配体结合过程中涉及的关键氨基酸位点，这为其结合特异性的分子相互作用提供了线索。我们的研究表明，AgerOBP23 可能成为未来研究 AgerOBP 配体结合的潜在靶标。这种方法符合反向化学生态学原理，为进一步探索 OBP 与各种化合物之间的分子相互作用，为未来的引诱剂或驱避剂开发研究奠定了基础。
