Bo Jingyi, Li Wen, Li Xiangyi, Li Zongbo, Mao Xiangzhong, Yang Bin, Zhao Ning
College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
Insects. 2025 May 23;16(6):553. doi: 10.3390/insects16060553.
In the chemosensory system of insects, odorant-binding proteins (OBPs) and odorant-degrading enzymes (ODEs) play a role in the host location process. This study identified and analyzed chemosensory-related genes from the transcriptomes of different tissues of male and female adults of . Subsequently, host odorants from and non-host odorants from were used to treat the adults of to clarify the gene expression changes in the insects and, combined with molecular docking, to explore the mechanism of the non-host odor interfering with the host localisation of . A total of 137 chemosensory-related genes were obtained, among which was specifically highly expressed in the antennae of ; and were highly expressed in the remnants of , selected as key genes for further research. The odor interference experiment results show that both host and non-host odorants caused up-regulation of in antennal expression, and significant changes in the expression of and in the remnants were also observed. The molecular docking results indicate that non-host compounds could compete with host compounds for protein binding sites. The non-host odor of can interfere with TyunOBPs and TyunCYPs in and affect their host localization.
在昆虫的化学感应系统中，气味结合蛋白（OBPs）和气味降解酶（ODEs）在宿主定位过程中发挥作用。本研究从[昆虫名称]雌雄成虫不同组织的转录组中鉴定并分析了化学感应相关基因。随后，用[昆虫名称]的宿主气味剂和非宿主气味剂处理[昆虫名称]成虫，以阐明昆虫体内基因表达的变化，并结合分子对接，探究非宿主气味干扰[昆虫名称]宿主定位的机制。共获得137个化学感应相关基因，其中[基因名称1]在[昆虫名称]触角中特异性高表达；[基因名称2]和[基因名称3]在[组织名称]残体中高表达，被选为进一步研究的关键基因。气味干扰实验结果表明，宿主和非宿主气味剂均导致触角中[基因名称4]表达上调，且在[组织名称]残体中也观察到[基因名称5]和[基因名称6]表达的显著变化。分子对接结果表明，非宿主化合物可与宿主化合物竞争蛋白质结合位点。[昆虫名称]的非宿主气味可干扰[昆虫名称]中的TyunOBPs和TyunCYPs，并影响其宿主定位。
