Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Menoufia University, Shibin El Kom, Egypt.
Department of Clinical Biochemistry, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia.
J Immunoassay Immunochem. 2024 Sep 2;45(5):432-451. doi: 10.1080/15321819.2024.2381524. Epub 2024 Jul 25.
The type I interferon (IFN) signature has been found to be overactivated in many systemic autoimmune diseases. This may be explained by impaired regulation of interferon-stimulated genes (ISGs) as well as interferon-induced protein 44 (IFI44) expression via their regulatory mechanisms via interferon regulatory factors (IRFs).
This case-control study includes two groups: 50 RA patients and 50 healthy controls. The quantification of IFI44 and IRF4 expression levels by the real-time PCR technique was estimated. Disease Activity Score-28 (DAS-28) was estimated for RA patients only.
Among the RA patients, there were statistically significant increased ESR, CRP, TLC, RF, and anti-CCP levels ( value < 0.001) and significant increased expression of the IFI44 and IRF4 genes ( value < 0.001). There was a significant positive correlation between the IFI44 and IRF4, and there was a significant correlation between both and ESR and anti-CCP among RA patients. At a cutoff point of 1.95, IFI44 shows higher sensitivity and specificity values than IRF4 for the diagnosis of RA.
IFI44 was more sensitive for RA diagnosis than IRF4. IFI44 and IRF4 overexpression could be promising predictors of RA diagnosis and might become useful clinical tools to guide therapeutic strategies.
在许多系统性自身免疫性疾病中发现 I 型干扰素（IFN）特征过度激活。这可能是由于干扰素刺激基因（ISGs）以及干扰素诱导蛋白 44（IFI44）的表达受到干扰素调节因子（IRFs）的调节机制的损害所致。
本病例对照研究包括两组：50 例 RA 患者和 50 例健康对照者。通过实时 PCR 技术估计 IFI44 和 IRF4 表达水平的定量。仅对 RA 患者估计疾病活动评分-28（DAS-28）。
在 RA 患者中，ESR、CRP、TLC、RF 和抗 CCP 水平显著升高（ 值<0.001），IFI44 和 IRF4 基因的表达显著升高（ 值<0.001）。在 RA 患者中，IFI44 和 IRF4 之间存在显著的正相关，两者与 ESR 和抗 CCP 之间存在显著相关性。在截点为 1.95 时，IFI44 对 RA 的诊断具有比 IRF4 更高的敏感性和特异性值。
IFI44 对 RA 的诊断比 IRF4 更敏感。IFI44 和 IRF4 的过度表达可能是 RA 诊断的有前途的预测因子，并可能成为指导治疗策略的有用临床工具。
