Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
J Infect Dis. 2024 Jul 25;230(1):221-230. doi: 10.1093/infdis/jiae008.
Soluble inflammatory factors in the cerebrospinal fluid (CSF) of patients with neurosyphilis have been investigated with low-throughput technology. This study aimed to illustrate the characteristics of soluble factor profiles in CSF of patients with neurosyphilis.
We measured the concentrations of 45 cytokines, chemokines, and growth factors in CSF from 112 untreated syphilis cases, including latent syphilis (LS), asymptomatic neurosyphilis (ANS), meningeal neurosyphilis (MNS), meningovascular neurosyphilis (MVNS), paralytic dementia (PD), and ocular syphilis (OS).
Thirty-three differentially expressed soluble factors (DeSFs) were categorized into 3 clusters. DeSF scores of clusters 1 and 2 (DeSFS1 and DeSFS2) were positively correlated with elevated neopterin and neurofilament light subunit (NF-L) concentration, respectively. DeSF scores of cluster 3 were positively correlated with white blood cells, protein, NF-L, and neopterin. Patients with LS, ANS, and OS exhibited an overall lower abundance of DeSFs. Patients with PD exhibited significantly increased levels of clusters 1 and 3, and the highest total DeSF score, whereas patients with MNS and MVNS showed enhanced levels of cluster 2. Receiver operating characteristic analysis revealed that DeSFS1 effectively discriminated PD, and DeSFS2 discriminated MNS/MVNS with high accuracy.
Patients with neurosyphilis at different stages have distinctive patterns of soluble factors in CSF, which are correlated with immune status and neuronal damage.
人们曾使用低通量技术研究神经梅毒患者脑脊液（CSF）中的可溶性炎症因子。本研究旨在阐明神经梅毒患者 CSF 中可溶性因子谱的特征。
我们测量了 112 例未经治疗的梅毒患者（包括潜伏梅毒[LS]、无症状神经梅毒[ANS]、脑膜神经梅毒[MNS]、脑膜血管神经梅毒[MVNS]、麻痹性痴呆[PD]和眼部梅毒[OS]）CSF 中的 45 种细胞因子、趋化因子和生长因子的浓度。
33 个差异表达的可溶性因子（DeSFs）被分为 3 个聚类。聚类 1 和 2 的 DeSF 评分（DeSFS1 和 DeSFS2）分别与升高的新蝶呤和神经丝轻链（NF-L）浓度呈正相关。聚类 3 的 DeSF 评分与白细胞、蛋白、NF-L 和新蝶呤呈正相关。LS、ANS 和 OS 患者的 DeSF 总体含量较低。PD 患者的聚类 1 和 3 水平显著升高，总 DeSF 评分最高，而 MNS 和 MVNS 患者的聚类 2 水平升高。受试者工作特征分析显示，DeSFS1 可有效区分 PD，DeSFS2 可准确区分 MNS/MVNS。
不同阶段的神经梅毒患者 CSF 中的可溶性因子具有独特的模式，与免疫状态和神经元损伤相关。
