ETH Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland.
ETH Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland.
Curr Opin Genet Dev. 2024 Aug;87:102231. doi: 10.1016/j.gde.2024.102231. Epub 2024 Jul 24.
Regenerative capacities and strategies vary dramatically across animals, as well as between cell types, organs, and with age. In recent years, high-throughput single-cell transcriptomics and other single-cell profiling technologies have been applied to many animal models to gain an understanding of the cellular and molecular mechanisms underlying regeneration. Here, we review recent single-cell studies of regeneration in diverse contexts and summarize key concepts that have emerged. The immense regenerative capacity of some invertebrates, exemplified by planarians, is driven mainly by the differentiation of abundant adult pluripotent stem cells, whereas in many other cases, regeneration involves the reactivation of embryonic or developmental gene-regulatory networks in differentiated cell types. However, regeneration also differs from development in many ways, including the use of regeneration-specific cell types and gene regulatory networks.
再生能力和策略在动物之间以及细胞类型、器官之间以及随年龄的变化而有很大的差异。近年来，高通量单细胞转录组学和其他单细胞分析技术已应用于许多动物模型，以了解再生的细胞和分子机制。在这里，我们回顾了不同背景下再生的最近的单细胞研究，并总结了出现的关键概念。一些扁形动物（例如水螅）的巨大再生能力主要是由大量成年多能干细胞的分化驱动的，而在许多其他情况下，再生涉及分化细胞类型中胚胎或发育基因调控网络的重新激活。然而，再生在许多方面也不同于发育，包括使用再生特异性细胞类型和基因调控网络。
