Department of Neurology, The First Affiliated Hospital of Dalian Medical University, Dalian City, Liaoning Province, China.
Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian City, Liaoning Province, China; Chang Zhou Hospital affiliated to Nanjing University of Chinese Medicine, Changzhou City, Jiangsu Province, China.
Acta Histochem. 2024 Oct;126(5-7):152184. doi: 10.1016/j.acthis.2024.152184. Epub 2024 Jul 24.
There is an urgent need for new treatments to solve hair loss problem. As mesenchymal stem cells were proved to have effects on promoting tissue repair and regeneration, in which the exosome plays a vital role, we aim to investigate the influence of umbilical cord mesenchymal stem cells exosome (UCMSC-Exos) on hair growth and its mechanism.
The hUCMSC-Exos were extracted by ultracentrifugation. Primary fibroblasts were cultured with or without hUCMSC-Exos and cell proliferation was evaluated by CCK-8 assay. C57BL/6 mice model of depilation-induced hair regrowth was treated with either hUCMSC-Exos (200 μg/mL) or PBS on one side of the dorsal back. Real time quantitative PCR, flow cytometry analysis, immunohistochemistry and Immunofluorescent staining were used to analyze the regulative effect of hUCMSC-Exos on hair follicle stem/progenitor cells and Wnt/β-catenin pathway.
The proliferation of fibroblasts incubated with hUCMSC-Exos at the concentration of 200 μg/mL was greater than other groups. Treatment with hUCMSC-Exos resulted in rapid reentry into anagen. Hair follicle stem/progenitor cell markers (K15, Lgr5, Lgr6, CD34 and Lrig1) and Wnt/β-catenin pathway related factors (Wnt5, Lef1, Lrp5 and β-catenin) were increased in hUCMSC-Exos-injected region.
hUCMSC-Exos promote fibroblasts proliferation and accelerate mouse hair regrowth by upregulating hair follicle stem/progenitor cell and Wnt/β-catenin pathway, which suggests potential therapeutic approaches for hair loss disorders.
急需新的治疗方法来解决脱发问题。间充质干细胞已被证明具有促进组织修复和再生的作用，其中外泌体起着至关重要的作用，我们旨在研究脐带间充质干细胞外泌体（UCMSC-Exos）对毛发生长的影响及其机制。
通过超速离心法提取 hUCMSC-Exos。用 hUCMSC-Exos（200μg/ml）或 PBS 处理原代成纤维细胞，并通过 CCK-8 法评估细胞增殖。在 C57BL/6 小鼠去毛诱导的毛发生长模型的背部一侧用 hUCMSC-Exos（200μg/ml）或 PBS 处理。实时定量 PCR、流式细胞术分析、免疫组织化学和免疫荧光染色用于分析 hUCMSC-Exos 对毛囊干细胞/祖细胞和 Wnt/β-catenin 通路的调节作用。
浓度为 200μg/ml 的 hUCMSC-Exos 孵育的成纤维细胞增殖大于其他组。用 hUCMSC-Exos 处理导致快速进入生长期。毛囊干细胞/祖细胞标志物（K15、Lgr5、Lgr6、CD34 和 Lrig1）和 Wnt/β-catenin 通路相关因子（Wnt5、Lef1、Lrp5 和 β-catenin）在 hUCMSC-Exos 注射部位增加。
hUCMSC-Exos 通过上调毛囊干细胞/祖细胞和 Wnt/β-catenin 通路促进成纤维细胞增殖，加速小鼠毛发生长，这为脱发疾病的治疗提供了潜在的方法。
