Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Reprod Immunol. 2024 Sep;165:104290. doi: 10.1016/j.jri.2024.104290. Epub 2024 Jun 30.
Folliculogenesis is the process where follicles in the ovaries develop and eventually lead to ovulation. Any disruption to this process can cause premature ovarian failure. miR-326 is one of the microRNAs whose expression leads to Th17 production. Th17 activates the immune system to respond more vigorously, and by producing interlukins and cytokines causes inflammation and autoimmune disorders. Th17-induced inflammation and Th17/Treg imbalance can result in POF. This investigation took samples from 30 POF patients and 30 healthy people. The study utilized PCR to assess the expression levels of cytokines, specific transcription factor (ROR-γt), and miR-326. Additionally, ELISA was employed to analyze serum levels of IL-17, IL-21, IL-23. Furthermore, flow cytometry was utilized to determine the frequency of Th17. Compared to the control group, our results demonstrated a rise in the transcription factor RORɣt and a considerable rise in the frequency of Th17 cells in patients with POF. The level of inflammatory cytokines IL-17, IL-21, and IL-23 secreted in serum samples of patients with POF increased significantly compared to the control group. Results of investigating microRNA associated with Th17 cells also showed increased expression of miR-326 in females suffering from POF. The elevation of pro-inflammatory markers in women with POF contrary to the control group underscores the significant involvement of the immune system in pregnancy disorders pathogenesis. Consequently, immunological factors may serve as promising biomarkers for predicting POF likelihood in high-risk women in the future.
卵泡发生是指卵巢中的卵泡发育并最终导致排卵的过程。这个过程中的任何干扰都可能导致卵巢早衰。miR-326 是一种 microRNA，其表达会导致 Th17 的产生。Th17 激活免疫系统以更强烈地响应，并通过产生白细胞介素和细胞因子引起炎症和自身免疫性疾病。Th17 诱导的炎症和 Th17/Treg 失衡可能导致 POF。这项研究从 30 名 POF 患者和 30 名健康人身上采集了样本。该研究利用 PCR 评估了细胞因子、特定转录因子（ROR-γt）和 miR-326 的表达水平。此外，还利用 ELISA 分析了血清中 IL-17、IL-21 和 IL-23 的水平。此外，还利用流式细胞术来确定 Th17 的频率。与对照组相比，我们的结果表明 POF 患者的转录因子 RORɣt 升高，Th17 细胞的频率显著升高。POF 患者血清样本中分泌的炎症细胞因子 IL-17、IL-21 和 IL-23 的水平与对照组相比显著升高。与 Th17 细胞相关的 microRNA 调查结果也表明，POF 女性的 miR-326 表达增加。POF 女性中促炎标志物的升高与对照组相比，强调了免疫系统在妊娠障碍发病机制中的重要作用。因此，免疫因素可能成为未来预测高危女性 POF 可能性的有前途的生物标志物。
