Department of Immunology, College of Basic Medical Sciences, Dalian Medical University, China.
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, China.
Int Immunopharmacol. 2024 Sep 30;139:112738. doi: 10.1016/j.intimp.2024.112738. Epub 2024 Jul 24.
Rheumatoid arthritis, a condition characterized by inflammation, has a substantial influence on both the worldwide economy and public health. Prior studies indicate that probiotics have the potential to enhance the composition of gut microbiota in instances of intestinal dysbiosis resulting from different disorders and contribute to the regulation of inflammation. The objective of this study is to investigate the impact of Saccharomyces boulardii on the gut microbiome in arthritis and its implications on inflammation.
The study utilized the Collagen Induced Arthritis (CIA) Sprague-Dawley (SD) rat model. After administering Saccharomyces boulardii (150 mg/kg/day) six days a week and Methotrexate (MTX) (0.2 mg/week) treatment for eight weeks, microbial DNA from the feces was sequenced using 16S rRNA. The evaluation of histopathology, bone loss, and cartilage degradation was conducted using histology, immunohistology assays, and micro-computed tomography (µCT) examinations. The enzyme-linked immunosorbent assay (ELISA) was used to analyze proinflammatory cytokines, while the western blot technique was applied to detect protein in the gut and in cell lines. The quantification of gene expression in gut,joint and cell lines was performed using real-time polymerase chain reaction. The cell lines were activated and then treated with the culture supernatant of S. boulardii for an in vitro investigation. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was utilized to assess cell proliferationand viability. Cellular motility was measured in a wound healing experiment, whereas apoptotic proteins were analyzed using Western blotting.
S. boulardii has been found to enhance bone and joint integrity, modulate gut microbiota, and mitigate proinflammatory cytokine levels in rats with arthritis. It decreases the permeability of the intestines and promotes the production of gut tight-junction proteins. The administration of S. boulardii inhibits the proliferation of T-helper-17 (Th17) and Type 3 innate lymphoid cells (ILC3). Additionally, it elicits apoptosis in MH7A cell lines and hinders their migratory activity.
This study provides valuable insights into the therapeutic potential of S. boulardii for treating and preventing arthritis in rats with collagen-induced arthritis by modulating gut microbiota and inflammation.
类风湿性关节炎是一种以炎症为特征的疾病，它对全球经济和公共卫生都有重大影响。先前的研究表明，益生菌有可能增强因不同疾病导致的肠道菌群失调而引起的肠道微生物群落组成，并有助于调节炎症。本研究旨在探讨酿酒酵母对关节炎肠道微生物组的影响及其对炎症的影响。
本研究采用胶原诱导关节炎（CIA）Sprague-Dawley（SD）大鼠模型。每周六天给予酿酒酵母（150mg/kg/天），每周一次给予甲氨蝶呤（MTX）（0.2mg/周）治疗八周后，采用 16S rRNA 测序分析粪便中的微生物 DNA。采用组织学、免疫组织化学检测和微计算机断层扫描（µCT）检查评估组织病理学、骨丢失和软骨降解。采用酶联免疫吸附试验（ELISA）分析促炎细胞因子，采用蛋白质印迹技术检测肠道和细胞系中的蛋白质。采用实时聚合酶链反应（PCR）定量检测肠道、关节和细胞系中的基因表达。采用细胞系激活和酿酒酵母培养上清液处理进行体外研究。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验评估细胞增殖和活力。采用划痕愈合实验测量细胞迁移能力，采用 Western blot 分析分析凋亡蛋白。
研究发现，酿酒酵母可增强关节炎大鼠的骨骼和关节完整性，调节肠道微生物群，并降低促炎细胞因子水平。它降低了肠道的通透性并促进了肠道紧密连接蛋白的产生。给予酿酒酵母可抑制辅助性 T 细胞 17（Th17）和 3 型固有淋巴细胞（ILC3）的增殖。此外，它可诱导 MH7A 细胞系凋亡并阻止其迁移活性。
本研究通过调节肠道微生物群和炎症，为酿酒酵母治疗和预防胶原诱导关节炎大鼠关节炎提供了有价值的见解。
