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[Application of lytic enzymes for disruption of microbial cell walls to isolate DNA].

作者信息

Shurygin A Ia, Zlishcheva E I, Rozhenko T G, Vorotilo S P

出版信息

Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):699-701.

PMID:390533
Abstract

The possibility of isolating DNA from yeast and actinomycete cells, previously exposed to enzymic disruption, was explored. The enzyme was produced from Actinomyces griseinus II. The highest yield of DNA from microbial cells was obtained as a result of detergent pretreatment of the yeast and actinomycetes and subsequent disruption of cell walls with the above enzyme.

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