Terasawa E
Endocrinology. 1985 Dec;117(6):2490-7. doi: 10.1210/endo-117-6-2490.
To examine developmental changes in the LH response to estrogen, eight neonatally ovariectomized monkeys received repeated injections (sc) of 50 micrograms/kg estradiol benzoate (EB) at approximately 4-month intervals starting at age 8-12 months and ending at 49-52 months. Serum samples were obtained 24 before and 0, 6, 12, 24, 36, 48, 60, 72, 94, 108, and 120 h after each EB injection. Serum LH and estradiol levels were measured by RIA. The baseline LH level before EB injection during the prepubertal period (greater than 20 months of age) was 14.4 +/- 2.2 ng/ml, and it increased progressively to 115.3 +/- 13.5 ng/ml at 41-44 months, the age shortly before the first ovulation in our intact colony animals, then declined slightly. EB first induced a typical LH response, which consisted of a negative phase (suppression) followed by a positive phase (surge), at the average age of 29.3 +/- 1.9 months (n = 8). This is similar to the age of menarche in our colony animals. The baseline LH level before EB injection at the time of the first typical response (with negative and positive phases) was 36.7 +/- 6.7 ng/ml, a level 2.5 times higher than that of the prepubertal age. The magnitude of LH suppression by EB was significantly correlated with the baseline level of LH; the higher the baseline LH before EB injection, the greater the degree of LH decrease (r = 0.968; P less than 0.001). Similarly, the amplitude of the LH peak from the trough of the negative phase was significantly correlated with the baseline LH; the higher the LH level before EB injection, the higher the LH increase (r = 0.863; P less than 0.001). The latency to the LH peak was shortest when baseline LH was highest; the peak latency (34.4 +/- 1.6 h) of the LH surge at 41-44 months of age was significantly shorter than the latency (46.5 +/- 2.7 h) of the first LH response occurring at 29.3 +/- 1.9 months of age (P less than 0.001). Finally, the pattern of circulating levels of estradiol after EB injection did not differ across the developmental stages examined. These results are interpreted to mean that an increase in LH release, presumably LHRH release, starts at the onset of puberty and continues until the age of first ovulation, and that the levels of LHRH release during the pubertal period may determine the effectiveness of estrogen on the LH surge.(ABSTRACT TRUNCATED AT 400 WORDS)
为研究促黄体生成素（LH）对雌激素反应的发育变化，8只新生期卵巢切除的猴子从8 - 12个月龄开始，每隔约4个月皮下重复注射50微克/千克苯甲酸雌二醇（EB），直至49 - 52个月龄。每次EB注射前24小时及注射后0、6、12、24、36、48、60、72、94、108和120小时采集血清样本。采用放射免疫分析法（RIA）测定血清LH和雌二醇水平。青春期前（大于20个月龄）EB注射前的LH基线水平为14.4±2.2纳克/毫升，在41 - 44个月龄时逐渐升高至115.3±13.5纳克/毫升，此为我们完整群体动物首次排卵前不久的年龄，之后略有下降。EB首次诱导典型的LH反应，包括负相（抑制）随后是正相（高峰），平均年龄为29.3±1.9个月（n = 8）。这与我们群体动物的初潮年龄相似。首次典型反应（有负相和正相）时EB注射前的LH基线水平为36.7±6.7纳克/毫升，比青春期前年龄的水平高2.5倍。EB对LH的抑制程度与LH基线水平显著相关；EB注射前LH基线越高，LH下降程度越大（r = 0.968；P < 0.001）。同样，从负相谷值到LH峰值的幅度与LH基线显著相关；EB注射前LH水平越高，LH升高幅度越大（r = 0.863；P < 0.001）。当基线LH最高时，LH峰值的潜伏期最短；41 - 44个月龄时LH高峰的峰值潜伏期（34.4±1.6小时）显著短于29.3±1.9个月龄时首次LH反应的潜伏期（46.5±2.7小时）（P < 0.001）。最后，在检查的不同发育阶段，EB注射后雌二醇的循环水平模式没有差异。这些结果被解释为意味着LH释放增加，可能是促性腺激素释放激素（LHRH）释放增加，始于青春期开始并持续到首次排卵年龄，并且青春期期间LHRH释放水平可能决定雌激素对LH高峰的作用效果。（摘要截断于400字）
