Terasawa E, Krook C, Eman S, Watanabe G, Bridson W E, Sholl S A, Hei D L
Endocrinology. 1987 Jun;120(6):2265-71. doi: 10.1210/endo-120-6-2265.
LH pulses during the progesterone (P)-induced LH surge were examined in ovariectomized and estrogen-treated female monkeys. Animals received a 2.5-mg P or oil injection 24 h after administration of 30 micrograms estradiol benzoate. The animals were fitted with jugular catheters connected to a tether-swivel system. Blood samples were collected at 10-min intervals starting 3-4 h before and ending 12-20 h after P or oil injection. Plasma LH was measured by both bioassay and RIA. LH pulses were determined by the PULSAR program. P administration induced a BIA-LH surge with a latency of 71 +/- 10 min in all seven animals. The P-induced bioassayable LH (BIA-LH) surge consisted of an ascending phase (204 +/- 24 min), a plateau period (174 +/- 32 min), and a descending phase (376 +/- 60 min). Oil injection did not cause a LH surge (n = 4). BIA-LH release before P and that during the P-induced LH surge were pulsatile. Pulse intervals of BIA-LH before P treatment (57.1 +/- 5.2 min) were not different from those before (55.0 +/- 11.7 min) and after (62.9 +/- 16.3 min) oil injection. In contrast, pulse intervals during the ascending phase (35.0 +/- 4.0 min), plateau period (34.6 +/- 2.6 min), and descending phase (45.0 +/- 3.1 min) were significantly shorter (P less than 0.02) than those before P. Pulse amplitudes of BIA-LH during the ascending phase (125.3 +/- 28.7 ng/ml) and plateau period (253.9 +/- 27.0 ng/ml) were significantly (P less than 0.001) higher than those (44.7 +/- 12.6 ng/ml) before P and during the descending phase (66.9 +/- 11.1 ng/ml). Radioimmunoassayable LH results were quite similar to those for BIA-LH, except that amplitude changes in radioimmunoassayable LH after P treatment were smaller than those in BIA-LH. It was concluded, therefore, that both the frequency and amplitude of pulsatile LH release increase during the P-induced LH surge, especially during the ascending phase and plateau period, in female rhesus monkeys. Furthermore, the present results support our previous conclusion that P facilitates pulsatile LHRH release with increases in frequency and amplitude in ovariectomized and estrogen-treated monkeys.
在切除卵巢并用雌激素处理的雌性猴子中，研究了孕酮（P）诱导促黄体生成素（LH）激增期间的LH脉冲。动物在给予30微克苯甲酸雌二醇24小时后接受2.5毫克P或油注射。给动物安装连接到系绳旋转系统的颈静脉导管。在P或油注射前3 - 4小时开始，以10分钟间隔采集血样，直至注射后12 - 20小时结束。通过生物测定法和放射免疫分析法（RIA）测量血浆LH。通过PULSAR程序确定LH脉冲。在所有7只动物中，P给药诱导了生物测定法测定的LH（BIA - LH）激增，潜伏期为71±10分钟。P诱导的生物测定法可检测到的LH（BIA - LH）激增包括一个上升期（204±24分钟）、一个平台期（174±32分钟）和一个下降期（376±60分钟）。注射油未引起LH激增（n = 4）。P给药前和P诱导的LH激增期间的BIA - LH释放是脉冲式的。P治疗前BIA - LH的脉冲间隔（57.1±5.2分钟）与注射油前（55.0±11.7分钟）和注射油后（62.9±16.3分钟）的脉冲间隔无差异。相比之下，上升期（35.0±4.0分钟）、平台期（34.6±2.6分钟）和下降期（45.0±3.1分钟）的脉冲间隔明显短于P给药前（P < 0.02）。上升期（125.3±28.7纳克/毫升）和平台期（253.9±27.0纳克/毫升）的BIA - LH脉冲幅度明显高于P给药前（44.7±12.6纳克/毫升）和下降期（66.9±11.1纳克/毫升）（P < 0.001）。放射免疫分析法可检测到的LH结果与BIA - LH的结果非常相似，只是P治疗后放射免疫分析法可检测到的LH的幅度变化小于BIA - LH。因此得出结论，在雌性恒河猴中，P诱导的LH激增期间，尤其是在上升期和平台期，脉冲式LH释放的频率和幅度均增加。此外，目前的结果支持我们之前的结论，即P促进切除卵巢并用雌激素处理的猴子中脉冲式促性腺激素释放激素（LHRH）的释放，频率和幅度均增加。
