School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
J Ethnopharmacol. 2024 Dec 5;335:118626. doi: 10.1016/j.jep.2024.118626. Epub 2024 Jul 23.
Banxia Xiexin Decoction (BXD) is one of the seven classic prescriptions of the special decoction method (SDM) of "removing dregs and decocting again", which has been widely used in inflammatory bowel diseases such as ulcerative colitis (UC). However, the impacts of SDM have not been fully investigated, either on the components or on the biological effects.
This study aimed to investigate the rational of SDM traditionally recorded about BXD, re-decoction after dreg-removal, by comparing with the contemporary general decoction method (GDM) from the perspective of phase states, in the bioactive components from the perspective of phase states, and their corresponding pharmacodynamic effects on a particular UC rat model.
The BXD decoctions were respectively obtained by SDM and GDM, together with the different samples with different decocting time. The phase state samples (true solution, colloidal phase, and precipitated phase), were also obtained after a series of separation process and characterized. The multi-components in the in-process decoctions, original decoctions and phase state samples were quantitatively determined. HPLC fingerprint spectrum of the samples were also detected and compared with chemometrics analysis. A rat model of ulcerative colitis with cold-heat complex syndrome was established, on which the pharmacodynamic effects of different phases of SDM-made BXD were investigated.
The results showed that the contents of eight marker components in SDM-made decoction were significantly higher than those in GDM-made decoction. Compared with the precipitated phases and true solutions, the colloidal phase was confirmed to obtain absolutely higher contents of the components (except berberine). The analysis on HPLC fingerprints also revealed that the profiles of colloidal phase showed the majority of the characteristics of original decoctions, when compared with the other phases. The results showed the BXD group, precipitated phase group and colloidal phase group had certain therapeutic effects on the ulcerative colitis rats with cold-heat complex syndrome, among which the original decoction group showed optimal effects, followed by the colloidal phase.
The study has provided the experimental evidence of the bioactive components and pharmacodynamic effects on the rational of SDM, as originally recorded about the classic prescription, which might provide useful idea for the interpretation on medicinal properties of TCM compound prescriptions, and contemporary TCM innovative drug developments.
半夏泻心汤（BXD）是“去渣再煎”七大经典方剂之一，已广泛应用于溃疡性结肠炎（UC）等炎症性肠病。然而，特殊煎煮法（SDM）对成分或生物效应的影响尚未得到充分研究。
本研究旨在从相态角度比较半夏泻心汤的传统 SDM 与当代通用煎煮法（GDM），探讨传统 SDM 去渣再煎的合理性，并从相态和药效的角度研究其生物活性成分。
采用 SDM 和 GDM 分别煎煮 BXD 及其不同煎煮时间的不同样本，通过一系列分离过程得到相态样本（真溶液、胶体相和沉淀相），并对其进行表征。对中间煎煮液、原始煎煮液和相态样本中的多组分进行定量测定。同时检测了样品的 HPLC 指纹图谱，并进行了化学计量学分析。建立寒热复合证溃疡性结肠炎大鼠模型，考察 SDM 制备的 BXD 不同相的药效。
结果表明，SDM 制备的汤剂中八种标记成分的含量明显高于 GDM 制备的汤剂。与沉淀相和真溶液相比，胶体相绝对获得更高的成分含量（除小檗碱外）。HPLC 指纹图谱分析也表明，与其他相相比，胶体相的图谱显示出与原始汤剂的大多数特征。结果表明，BXD 组、沉淀相组和胶体相组对寒热复合证溃疡性结肠炎大鼠均有一定的治疗作用，其中原始汤剂组效果最佳，其次是胶体相。
本研究为经典方剂中记载的 SDM 的生物活性成分和药效提供了实验依据，为阐释中药复方的药性和开发现代中药创新药物提供了有益思路。
