培养的牛视网膜毛细血管周细胞对葡萄糖转运的特性研究。

Characterization of glucose transport by bovine retinal capillary pericytes in culture.

作者信息

Li W, Chan L S, Khatami M, Rockey J H

出版信息

Exp Eye Res. 1985 Aug;41(2):191-9. doi: 10.1016/0014-4835(85)90024-7.

Abstract

The transport of D-glucose and its non-metabolizable analog, 3-o-methyl-D-glucose (3-o-MG), by cultured bovine retinal capillary pericytes was characterized. A preference for D-glucose over L-glucose uptake by pericytes indicated a stereospecific process. The 3-o-MG transport appeared to follow complex kinetics. At low substrate concentrations, 3-o-MG transport had an apparent Km of 1.53 mM and a Vmax of 0.50 nmol (microgram DNA)-1 min-1 at 37 degrees C. A reduced extracellular sodium concentration, energy poisons, and exogenous insulin did not significantly influence 3-o-MG uptake. Inhibition of the uptake or efflux of 3-o-MG by cytochalasin B or phloretin, respectively, and the demonstration of a 'counter transport' phenomenon showed that 3-o-MG transport by retinal capillary pericytes in culture was a carrier-mediated process.

摘要

对培养的牛视网膜毛细血管周细胞转运D-葡萄糖及其非代谢类似物3-O-甲基-D-葡萄糖（3-O-MG）的特性进行了研究。周细胞对D-葡萄糖的摄取优先于L-葡萄糖，表明这是一个立体特异性过程。3-O-MG的转运似乎遵循复杂的动力学。在低底物浓度下，37℃时3-O-MG转运的表观Km为1.53 mM，Vmax为0.50 nmol（微克DNA）-1 分钟-1。细胞外钠浓度降低、能量毒物和外源性胰岛素对3-O-MG的摄取没有显著影响。细胞松弛素B或根皮素分别抑制3-O-MG的摄取或外排，以及“反向转运”现象的证明表明，培养的视网膜毛细血管周细胞对3-O-MG的转运是一个载体介导的过程。