College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, People's Republic of China.
The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou, People's Republic of China.
Biomed Chromatogr. 2024 Oct;38(10):e5961. doi: 10.1002/bmc.5961. Epub 2024 Jul 25.
Gardeniae fructus (GF) is known for its various beneficial effects on cholestatic liver injury (CLI). However, the biological mechanisms through which GF regulates CLI have not been fully elucidated. This study aimed to explore the potential mechanisms of GF against α-naphthylisothiocyanate (ANIT)-induced CLI. First, HPLC technology was used to analyze the chemical profile of the GF extract. Second, the effects of GF on serum biochemical indicators and liver histopathology were examined. Lastly, metabolomics was utilized to study the changes in liver metabolites and clarify the associated metabolic pathways. In chemical analysis, 10 components were identified in the GF extract. GF treatment regulated serum biochemical indicators in ANIT-induced CLI model rats and alleviated liver histological damage. Metabolomics identified 26 endogenous metabolites as biomarkers of ANIT-induced CLI, with 23 biomarkers returning to normal levels, particularly involving primary bile acid biosynthesis, glycerophospholipid metabolism, tryptophan metabolism, and arachidonic acid metabolism. GF shows promise in alleviating ANIT-induced CLI by modulating multiple pathways.
栀子（GF）以其对胆汁淤积性肝损伤（CLI）的多种有益作用而闻名。然而，GF 调节 CLI 的生物学机制尚未完全阐明。本研究旨在探讨 GF 对α-萘异硫氰酸酯（ANIT）诱导的 CLI 的潜在作用机制。首先，采用高效液相色谱技术分析 GF 提取物的化学成分。其次，观察 GF 对血清生化指标和肝组织病理学的影响。最后，利用代谢组学研究肝代谢物的变化并阐明相关代谢途径。在化学分析中，GF 提取物中鉴定出 10 种成分。GF 处理可调节 ANIT 诱导的 CLI 模型大鼠的血清生化指标，并减轻肝组织损伤。代谢组学鉴定出 26 种内源性代谢物作为 ANIT 诱导的 CLI 的生物标志物，其中 23 种标志物恢复正常水平，特别是涉及初级胆汁酸生物合成、甘油磷脂代谢、色氨酸代谢和花生四烯酸代谢。GF 通过调节多种途径缓解 ANIT 诱导的 CLI 具有潜力。
