Jang Hyun-Jun, Kim Hyun-Yong, Lyu Ji Hyo, Muthamil Subramanian, Shin Ung Cheol, Kim Hyo Seon, Jeong Jieun, Chang Suwhan, Lee Yun Kyung, Park Jun Hong
Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine Naju Korea.
Research Group of Personalized Diet Korea Food Research Institute Wanju-gun Korea.
Food Sci Nutr. 2024 Apr 17;12(7):5027-5035. doi: 10.1002/fsn3.4153. eCollection 2024 Jul.
Cachexia is associated with various diseases, such as heart disease, infectious disease, and cancer. In particular, cancer-associated cachexia (CAC) accounts for more than 20% of mortality in cancer patients worldwide. Adipose tissue in CAC is characterized by adipocyte atrophy, mainly due to excessively increased lipolysis and impairment of adipogenesis. CAC is well known for the loss of skeletal muscle mass and/or fat mass. CAC induces severe metabolic alterations, including protein, lipid, and carbohydrate metabolism. The objectives of this study were to evaluate the effects of bee wax ( L. 1758) (BW) extract on adipogenesis, lipolysis, and mitochondrial oxygen consumption through white adipocytes, 3T3-L1. To achieve this study, cancer-associated cachexia condition was established by incubation of 3T3-L1 with colon cancer cell line CT26 cultured media. BW extract recovered the reduced adipogenesis under cachectic conditions in CT26 media. Treatment of BW showed increasing lipid accumulation as well as adipogenic gene expression and its target gene during adipogenesis. The administration of BW to adipocytes could decrease lipolysis. Also, BW could significantly downregulated the mitochondrial fatty acid oxidation-related genes, oxygen consumption rate, and extracellular acidification rate. Our results suggest that BW could improve metabolic disorders such as CAC through the activation of adipogenesis and inhibition of lipolysis in adipocytes, although we need further validation in vivo CAC model to check the effects of BW extract. Therefore, BW extract supplements could be useful as an alternative medicine to reverse energy imbalances.
恶病质与多种疾病相关，如心脏病、传染病和癌症。特别是，癌症相关性恶病质（CAC）占全球癌症患者死亡率的20%以上。CAC中的脂肪组织以脂肪细胞萎缩为特征，主要是由于脂解过度增加和成脂受损。CAC以骨骼肌质量和/或脂肪质量的丧失而闻名。CAC会引发严重的代谢改变，包括蛋白质、脂质和碳水化合物代谢。本研究的目的是通过白色脂肪细胞3T3-L1评估蜂蜡（L. 1758）（BW）提取物对成脂、脂解和线粒体氧消耗的影响。为了实现这项研究，通过将3T3-L1与结肠癌细胞系CT26培养基孵育来建立癌症相关性恶病质条件。BW提取物恢复了CT26培养基中恶病质条件下降低的成脂作用。BW处理显示在成脂过程中脂质积累增加以及成脂基因表达及其靶基因增加。将BW给予脂肪细胞可降低脂解作用。此外，BW可显著下调线粒体脂肪酸氧化相关基因、氧消耗率和细胞外酸化率。我们的结果表明，BW可通过激活成脂作用和抑制脂肪细胞中的脂解作用来改善诸如CAC等代谢紊乱，尽管我们需要在体内CAC模型中进一步验证以检查BW提取物的效果。因此，BW提取物补充剂可能作为一种替代药物来逆转能量失衡。
