通过CRISPR/Cas9介导的同源定向修复在……中实现高效敲入
Efficient Knock-in by CRISPR/Cas9-mediated homology-directed repair in .
作者信息
Ma Jiaonv, Zhou Wangyan, Shao Wenhua, Li Yongbin
机构信息
College of Life Science, Capital Normal University, Beijing, Beijing, China.
出版信息
MicroPubl Biol. 2024 Jul 10;2024. doi: 10.17912/micropub.biology.001203. eCollection 2024.
Abstract
The CRISPR/Cas9 technology opens new avenues for detailed genetic exploration and comparative genomics. However, the current application of CRISPR/Cas9 in especially for homology-directed repair (HDR) knock-in (KI) still encounter significant challenges. In this study, we demonstrate that employing high concentration HDR donor vectors significantly improves KI efficiency in .
摘要
CRISPR/Cas9技术为详细的基因探索和比较基因组学开辟了新途径。然而，CRISPR/Cas9目前在尤其是同源定向修复（HDR）敲入（KI）方面的应用仍然面临重大挑战。在本研究中，我们证明使用高浓度的HDR供体载体可显著提高[具体物种或细胞系未明确]中的KI效率。
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