用于农药检测的镀银聚苯乙烯微球阵列表面增强拉曼散射微流控芯片

Peng Wang, Xu Zhihan, Yi Chao, Zhang Yuankai, Liao Qingxi

College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China.

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, China.

Heliyon. 2024 Jun 26;10(13):e33647. doi: 10.1016/j.heliyon.2024.e33647. eCollection 2024 Jul 15.

Carbendazim and acetamidine are pesticides that widely used to control pests and diseases in oilseed rape. In this paper, a rapid, accurate and reliable method was proposed for the detection of carbendazim and acetamidine with SERS microfluidic chip technology. Ag-ps(Polystyrene microspheres) microsphere SERS substrate was prepared by spin coating and magnetron sputtering deposition of Ag. The enhancement factor of prepared SERS substrate was 2.4 × 10. The SERS detection working curves were well fitted and the linear parameters R were 0.987 and 0.994, respectively. The limit of detection was 0.01 mg/mL. The use of SERS microfluidic chip to detect carbendazim and acetamidine is expected to provide a way for the detection of pesticide residues in crops, which has broad application prospects in the field of food safety.

多菌灵和乙脒是广泛用于防治油菜病虫害的农药。本文提出了一种利用表面增强拉曼散射（SERS）微流控芯片技术快速、准确、可靠地检测多菌灵和乙脒的方法。通过旋涂和磁控溅射沉积银制备了Ag-ps（聚苯乙烯微球）微球SERS基底。制备的SERS基底的增强因子为2.4×10。SERS检测工作曲线拟合良好，线性参数R分别为0.987和0.994。检测限为0.01mg/mL。利用SERS微流控芯片检测多菌灵和乙脒有望为作物中农药残留的检测提供一种途径，在食品安全领域具有广阔的应用前景。