综合生理学分析、转录组和DNA甲基化揭示了梨柱头-花柱复合体发育调控的优势。

Integrated physiological analyses, transcriptome, and DNA methylation reveal superiority of pear stigma-style complex development regulation.

作者信息

Huang Xu, Zhai Li-Hua, Kong Xiao-Xiong, Zhang Jing, Liu Xiao, Wang Chun-Lei

机构信息

College of Horticulture and Landscape Architecture, International Research Laboratory of Agriculture and Agri-Product Safety, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, 48 Wenhui East Road, Yangzhou 225009, People's Republic of China.

出版信息

iScience. 2024 Jun 24;27(7):110372. doi: 10.1016/j.isci.2024.110372. eCollection 2024 Jul 19.

DOI:10.1016/j.isci.2024.110372

PMID:39055924

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11269929/

Abstract

Styles and stigmas are crucial components of the fertilization process that allows a pear tree to bear fruit. The information regarding the development mechanism of pear style and stigma is still unclear. Our results demonstrated that IAA, ABA, and BR are significantly increased at 1 DBF, while JA is decreased at 5 DBF. The fructose and starch contents significantly increased at 1 DBF when the style with stigma was ready for pollination. Transcriptome and DNA methylation analysis showed 8087 DEGs and 3771 DMRs were enriched in plant hormones biosynthesis, carbohydrate biosynthesis and metabolism, and TFs in 1 DBF as compared with 7 DBF. The CHH methylation type of DMRs accounts for 84.75%. Most DMRs of CHH upregulated in 1 DBF vs. 7 DBF. This study found for the first time that transcription factor ERFs and DNA methylation are involved in regulating the growth and development of fruit plant style and stigma.

摘要

花柱和柱头是梨树结果所需受精过程的关键组成部分。关于梨属花柱和柱头发育机制的信息仍不明确。我们的结果表明，吲哚 - 3 - 乙酸（IAA）、脱落酸（ABA）和油菜素内酯（BR）在开花后1天（DBF）显著增加，而茉莉酸（JA）在开花后5天（DBF）下降。当带有柱头的花柱准备好授粉时，果糖和淀粉含量在开花后1天显著增加。转录组和DNA甲基化分析表明，与开花后7天（DBF）相比，开花后1天有8087个差异表达基因（DEG）和3771个差异甲基化区域（DMR）富集在植物激素生物合成、碳水化合物生物合成与代谢以及转录因子中。DMR的CHH甲基化类型占84.75%。与开花后7天相比，CHH的大多数DMR在开花后1天上调。本研究首次发现转录因子ERF和DNA甲基化参与调控果树花柱和柱头的生长发育。