Nuclear enhances the malignant behavior of A549 cells and impacts lung adenocarcinoma patient prognosis.

is a metastatic suppressor inconsistently reported to have multiple roles as both a promoter and inhibitor of cancer metastasis. Nevertheless, the specific mechanism behind these results is still unclear. We observed that A549 cells with stable transfer of into the nucleus (A549-nNm23-H1) exhibited significantly increased migration and invasion activity compared to vector control cells, which was further enhanced by over-expressing CYP24A1 ( < 0.001). demonstrated the ability to safely attach to and amplify the transcription activation of JUN, consequently leading to the up-regulation of . Analysis of clinical data showed a positive relationship between nuclear levels and expression. Furthermore, they were positively associated with postoperative distant metastasis and negatively correlated with prognosis in those with early stage lung adenocarcinoma. In conclusion, the data presented provides a new understanding of the probable pathways by which nuclear facilitates tumor metastasis, establishing the groundwork for future prediction and treatment of tumor metastasis.