促炎细胞因子白细胞介素-21 与类风湿关节炎患者的活性氧和 25-羟维生素 D 相关。

Pro-inflammatory cytokine IL-21 correlates with the reactive oxygen species and 25-hydroxy vitamin D in rheumatoid arthritis patients.

机构信息

Rheumatology and Immunology Department, Fourth Central Hospital of Baoding City, Baoding, Hebei Province, China.

Obstetrics Department, Fourth Central Hospital of Baoding City, Baoding, Hebei Province, China.

出版信息

Immun Inflamm Dis. 2024 Jul;12(7):e1308. doi: 10.1002/iid3.1308.

DOI:10.1002/iid3.1308

PMID:39056553

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11273535/

Abstract

BACKGROUND

Rheumatoid arthritis (RA) is a chronic autoimmune disorder and its characteristics include the immune system's invasion of the healthy lining of the joints and the articular structures degeneration. The IL-21 pro-inflammatory cytokine, and the reactive oxygen species (ROS) might have a role in the RA etiopathogenesis. The present study assessed the correlation of IL-21 with vitamin 25(OH)D and the ROS.

METHODS

The study included 120 RA patients and 60 healthy group. The RA patients were categorized based on rheumatoid factor (RF) seropositivity or seronegativity and the RA severity. Chemiluminescent immunoassay and 10% hematocrit were used to check levels of vitamin 25(OH)D and ROS, respectively. ELISA was used for the detection of IL-21 in the plasma.

RESULTS

The RA patients had a significantly reduced vitamin 25(OH)D level compared to the healthy controls. The IL-21 and ROS were however significantly increased in the RA patients compared to the controls. Further, the seropositive RF and the high RA severity patients had significant IL-21 and ROS increase in comparison with the seronegative RF and the low severity RA patients. Finally, IL-21 negatively correlated with vitamin 25(OH)D, but positively correlated with the ROS.

CONCLUSION

This is the first investigation to confirm the relationship between IL-21 with vitamin 25(OH)D and the ROS among the RA patients. The findings indicate that vitamin 25(OH)D is reduced in the RA patients' serum. ROS and IL-21 are also associated with increased RA severity.

摘要

背景

类风湿关节炎（RA）是一种慢性自身免疫性疾病，其特征包括免疫系统侵犯关节健康衬里和关节结构退化。白细胞介素 21（IL-21）促炎细胞因子和活性氧物种（ROS）可能在 RA 的发病机制中起作用。本研究评估了 IL-21 与维生素 25（OH）D 和 ROS 的相关性。

方法

该研究纳入了 120 例 RA 患者和 60 名健康对照组。根据类风湿因子（RF）血清阳性或阴性以及 RA 严重程度对 RA 患者进行分类。化学发光免疫分析和 10%红细胞压积分别用于检测维生素 25（OH）D 和 ROS 的水平。ELISA 用于检测血浆中的 IL-21。

结果

与健康对照组相比，RA 患者的维生素 25（OH）D 水平显著降低。然而，与对照组相比，RA 患者的 IL-21 和 ROS 水平显著升高。此外，血清阳性 RF 和高严重程度 RA 患者的 IL-21 和 ROS 水平显著高于血清阴性 RF 和低严重程度 RA 患者。最后，IL-21 与维生素 25（OH）D 呈负相关，与 ROS 呈正相关。