Abrego-Guandique Diana Marisol, Ilori Olubukunmi Amos, Caroleo Maria Cristina, Cannataro Roberto, Cione Erika, Tucci Paola
Department of Health Sciences, University of Magna Graecia Catanzaro, 88100 Catanzaro, Italy.
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy.
Curr Issues Mol Biol. 2024 Jul 15;46(7):7473-7485. doi: 10.3390/cimb46070443.
Dietary microRNAs (miRs) represent a new area in food science. Although they have been found in many foods, including honey, more research is needed about their stability and fate during digestion. Hence, this study aimed to analyze the digestive stability of two selected miRs in honey. We extracted miR-92a-3p and miR-30c-5p from pasteurized and unpasteurized forms of polyfloral honey using two different methods and kits: a column-based manual method and a phenol-free semi-automated magnetic-bead-based method. The latter option was used for the subsequent analysis of samples according to the INFOGEST static digestion protocol. Also, the honey samples were examined for exosome-like particles using dynamic light scattering. Although the expression levels of both miRs were significantly lower following intestinal digestion, we found a difference in the resilience of the miRs to gastrointestinal conditions, with miR-30c-5p being relatively stable compared to miR-92a-3p following digestion, regardless of the honey's pasteurization treatment. Moreover, there was marked heterogeneity in the extracellular vesicle profile of the pasteurized sample. We identified the presence of two broadly conserved miRs in honey: miR-92a-3p and miR-30c-5p. Despite honey exhibiting high digestibility, miR-92a-3p was less resilient than miR-30c-5p, demonstrating considerable resistance under gastrointestinal conditions. Although further research is needed, the results obtained from this study may represent a starting point for utilizing honey as a source of exogenous miRNAs for preventive strategies and more "natural" treatments.
膳食微小RNA（miRs）是食品科学中的一个新领域。尽管它们已在包括蜂蜜在内的许多食物中被发现，但关于它们在消化过程中的稳定性和去向仍需要更多研究。因此，本研究旨在分析蜂蜜中两种选定miRs的消化稳定性。我们使用两种不同的方法和试剂盒，从巴氏杀菌和未巴氏杀菌的多花蜂蜜中提取miR-92a-3p和miR-30c-5p：基于柱的手动方法和无酚半自动磁珠法。根据INFOGEST静态消化方案，后一种方法用于后续样品分析。此外，使用动态光散射对蜂蜜样品进行外泌体样颗粒检测。尽管两种miRs在肠道消化后的表达水平均显著降低，但我们发现miRs对胃肠道条件的恢复能力存在差异，无论蜂蜜的巴氏杀菌处理如何，消化后miR-30c-5p相对于miR-92a-3p相对稳定。此外，巴氏杀菌样品的细胞外囊泡谱存在明显的异质性。我们在蜂蜜中鉴定出两种广泛保守的miRs：miR-92a-3p和miR-30c-5p。尽管蜂蜜具有高消化性，但miR-92a-3p的恢复能力不如miR-30c-5p，在胃肠道条件下表现出相当的抗性。尽管还需要进一步研究，但本研究获得的结果可能代表了将蜂蜜用作预防性策略和更“天然”治疗的外源性miRNAs来源的起点。
