McCann J P, Reimers T J
J Anim Sci. 1985 Sep;61(3):619-23. doi: 10.2527/jas1985.613619x.
This study determined if the insulin and glucose responses to glucose infusion in obese (n = 4) and lean (n = 4) Holstein heifers were affected by stage of the estrous cycle. Glucose (.35 g/kg) was infused within 2 min into the jugular veins of heifers during diestrus (d 15) and at the subsequent estrus (d 0). Concentrations of insulin and glucose were determined in jugular venous serum obtained from blood samples collected at 60, 45, 30, 15 and 1 min before and at 2.5, 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, 120, 140, 160, 180, 210 and 240 min after glucose. Mean (+/- SE) pretreatment concentrations of glucose (mg/100 ml) in obese (68 +/- 1.9) and lean (71 +/- 2.5) heifers were unaffected by body condition and stage of the cycle. Mean (+/- SE) pretreatment concentrations of insulin (microU/ml) were unaffected by stage of the cycle but were higher (P less than .05) in obese (33 +/- 3.6) than in lean (18 +/- 2.7) heifers. Body condition affected the insulin response with greater absolute concentrations (P less than .01) and total (P less than .005) response areas of insulin in obese than in lean heifers. Kinetics of the injected glucose were unaffected by body condition and stage of the cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在确定发情周期阶段是否会影响肥胖（n = 4）和瘦（n = 4）的荷斯坦小母牛对葡萄糖输注的胰岛素和葡萄糖反应。在间情期（第15天）和随后的发情期（第0天），在2分钟内将葡萄糖（0.35 g/kg）注入小母牛的颈静脉。在葡萄糖输注前60、45、30、15和1分钟以及输注后2.5、5、10、15、20、30、40、50、60、80、100、120、140、160、180、210和240分钟采集血样，测定颈静脉血清中的胰岛素和葡萄糖浓度。肥胖（68±1.9）和瘦（71±2.5）小母牛的葡萄糖预处理平均浓度（mg/100 ml）不受身体状况和周期阶段的影响。胰岛素预处理平均浓度（μU/ml）不受周期阶段的影响，但肥胖（33±3.6）小母牛的浓度高于瘦（18±2.7）小母牛（P<0.05）。身体状况影响胰岛素反应，肥胖小母牛的胰岛素绝对浓度（P<0.01）和总反应面积（P<0.005）均大于瘦小母牛。注入葡萄糖的动力学不受身体状况和周期阶段的影响。（摘要截短至250字）
