MOE Key Laboratory of Marine Genetics & Breeding and College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Mar Drugs. 2024 Jun 22;22(7):289. doi: 10.3390/md22070289.
Bacteria from the genus are distributed across various marine habitats and play a significant role in sulfur cycling. However, the metabolic features of inhabiting marine biofilms are still not well understood. Here, complete genomes and paired metatranscriptomes of eight strains, isolated from biofilms on subtidal stones, have been analyzed to explore their central energy metabolism and potential of secondary metabolite biosynthesis. Based on average nucleotide identity and phylogenetic analysis, the eight strains were classified into six novel species and two novel strains. The reconstruction of the metabolic pathways indicated that all strains had a complete Entner-Doudoroff pathway, pentose phosphate pathway, and diverse pathways for amino acid metabolism, suggesting the presence of an optimized central carbon metabolism. Pangenome analysis further revealed the differences between the gene cluster distribution patterns among the eight strains, suggesting significant functional variation. Moreover, a total of 47 biosynthetic gene clusters were discovered, which were further classified into 37 gene cluster families that showed low similarity with previously documented clusters. Furthermore, metatranscriptomic analysis revealed the expressions of key functional genes involved in the biosynthesis of ribosomal peptides in in situ marine biofilms. Overall, this study sheds new light on the metabolic features, adaptive strategies, and value of genome mining in this group of biofilm-associated bacteria.
属于 的细菌分布在各种海洋生境中，在硫循环中发挥着重要作用。然而，栖息在海洋生物膜中的 的代谢特征仍未得到很好的理解。在这里，我们分析了从潮间带石头生物膜中分离出的 8 株 菌株的完整基因组和配对的宏转录组，以探索它们的中心能量代谢和次生代谢产物生物合成的潜力。基于平均核苷酸同一性和系统发育分析，这 8 株菌被分为 6 个新种和 2 个新菌株。代谢途径的重建表明，所有菌株都具有完整的 Entner-Doudoroff 途径、戊糖磷酸途径和多种氨基酸代谢途径，表明存在优化的中心碳代谢。泛基因组分析进一步揭示了 8 株菌之间基因簇分布模式的差异，表明存在显著的功能变异。此外，共发现了 47 个生物合成基因簇，进一步分为 37 个基因簇家族，与先前记录的簇的相似度较低。此外，宏转录组分析揭示了原位海洋生物膜中参与核糖体肽生物合成的关键功能基因的表达情况。总的来说，本研究揭示了这组与生物膜相关的 细菌的代谢特征、适应策略以及基因组挖掘的价值。
