Backhaus Joy, Kann Simone, Hahn Andreas, Weinreich Felix, Blohm Martin, Tanida Konstantin, Feldt Torsten, Sarfo Fred Stephen, Di Cristanziano Veronica, Loderstädt Ulrike, Ehrhardt Stephan, Schoppen Stefanie, Tagbor Harry, Frickmann Hagen, Eberhardt Kirsten Alexandra
Statistical Consulting, 97074 Wuerzburg, Germany.
Department of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, Germany.
Pathogens. 2024 Jul 15;13(7):583. doi: 10.3390/pathogens13070583.
The study was conducted to identify cluster patterns of enteric microorganisms with potential etiological relevance for infectious gastroenteritis in stool samples of individuals from Ghana, which is a known high-endemicity setting for infectious gastroenteritis. These patterns were compared to previous observations with specimens from Colombian indigenous people in order to assess potentially stable clustering for temporally and spatially distinct populations from high-endemicity regions. By doing so, the study aimed to identify stable clusters as markers of microbial interaction with potential importance for etiological relevance assignment in cases of multiple enteric pathogen detections. Stool samples from 1569 Ghanaian individuals (875 from HIV patients, 30 from HIV-negative control adult patients, and 644 from children < 2 years of age) were assessed for enteric microorganisms by applying real-time PCR. As a result, nucleic acids of bacterial microorganisms were most frequently detected, followed by protozoa, microsporidia, and helminths. Interestingly, the cluster assessment confirmed interaction patterns known from the previous analysis with Colombian indigenous people, demonstrating a high likelihood of for clustering with other microorganisms and a prominent, potentially mediating role of for microbial interactions within the clusters. In conclusion, the assessment confirmed conserved clustering of enteric microorganisms with potential etiological relevance for human infectious gastroenteritis over geographically distinct high-endemicity settings. Furthermore, the composition of abundant microorganisms is more important than regional factors for the determination of the interplay of enteric microorganisms in the human gut. Thereby, some microbial pathogens and commensals seem more susceptible to a changing microbial composition in the human gut than others.
开展这项研究是为了在来自加纳的个体粪便样本中识别与感染性肠胃炎具有潜在病因学关联的肠道微生物群集模式，加纳是已知的感染性肠胃炎高流行地区。将这些模式与之前对哥伦比亚原住民样本的观察结果进行比较，以评估来自高流行地区的时间和空间上不同人群中潜在稳定的群集情况。通过这样做，该研究旨在识别稳定的群集，作为微生物相互作用的标志物，这对于在检测到多种肠道病原体的情况下进行病因学关联分配可能具有重要意义。通过实时PCR对1569名加纳个体（875名来自HIV患者，30名来自HIV阴性对照成年患者，644名来自2岁以下儿童）的粪便样本进行肠道微生物评估。结果，细菌微生物的核酸最常被检测到，其次是原生动物、微孢子虫和蠕虫。有趣的是，群集评估证实了之前对哥伦比亚原住民分析中已知的相互作用模式，表明与其他微生物群集的可能性很高，并且在群集内微生物相互作用中发挥着突出的、潜在的介导作用。总之，评估证实了在地理上不同的高流行地区，与人类感染性肠胃炎具有潜在病因学关联的肠道微生物存在保守的群集情况。此外，对于确定人类肠道中肠道微生物的相互作用而言，丰富微生物的组成比区域因素更重要。因此，一些微生物病原体和共生菌似乎比其他微生物更容易受到人类肠道中不断变化的微生物组成的影响。
