Gothe Julia, Horn Matthias, Baums Christoph G, Heilmann Romy M, Schrödl Wieland
Institute of Bacteriology and Mycology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, 04103 Leipzig, Germany.
Institute for Medical Informatics, Statistics and Epidemiology (IMISE), Faculty of Medicine, University of Leipzig, 04107 Leipzig, Germany.
Vet Sci. 2024 Jul 3;11(7):302. doi: 10.3390/vetsci11070302.
() is a pathogen in pigs, poultry, and humans causing colitis, diarrhea, and poor growth rates. Its role as a canine pathogen is controversial, and the seroprevalence of specific IgG antibodies against in dogs is unknown. A further, not yet officially recognized species in dogs is "" (""), which is proposed to be apathogenic. This study evaluates enzyme-linked immunosorbent assays (ELISAs) measuring serum IgG antibodies specific for or "" and investigates levels of specific IgG antibodies against and "" in a cohort of clinical patients presented at an animal referral clinic. These ELISAs use detergent-extracted antigens from and "". To increase analytic specificity, we precipitated the antigens with trichloroacetic acid (TCA) to isolate and concentrate the respective protein fraction. Our results indicate that a large number of serum IgG antibodies bind to shared epitopes of detergent-extracted antigens of the two spirochaetes. Our data also suggest that dogs might not only carry but also have ""-specific serum IgG antibodies.
（某病原体）是猪、家禽和人类的一种病原体，可导致结肠炎、腹泻和生长速度缓慢。其作为犬类病原体的作用存在争议，犬类中针对该病原体的特异性IgG抗体的血清阳性率尚不清楚。犬类中另一种尚未得到官方认可的该病原体物种是“某某（特定名称）”，有人认为它无致病性。本研究评估了检测针对该病原体或“某某”的血清IgG抗体的酶联免疫吸附测定（ELISA），并调查了在一家动物转诊诊所就诊的一组临床病犬中针对该病原体和“某某”的特异性IgG抗体水平。这些ELISA使用从该病原体和“某某”中用去污剂提取的抗原。为了提高分析特异性，我们用三氯乙酸（TCA）沉淀抗原，以分离和浓缩各自的蛋白质部分。我们的结果表明，大量血清IgG抗体与这两种螺旋体的去污剂提取抗原的共同表位结合。我们的数据还表明，犬类可能不仅携带该病原体，还具有针对“某某”的特异性血清IgG抗体。
