Saputra Febriyansyah, Kishida Mitsuyo, Hu Shao-Yang
Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.
Toxics. 2024 Jul 18;12(7):518. doi: 10.3390/toxics12070518.
Nitrate and nitrite have emerged as increasingly common environmental pollutants, posing significant risks to various forms of life within ecosystems. To understand their impact on the visual system of zebrafish, adult zebrafish were exposed to environmentally relevant concentrations of nitrate (10 mg/L) and nitrite (1 mg/L) for 7 days. Visual behaviors were examined using optomotor and avoidance response. The eyeballs of the zebrafish were collected for H&E staining, IHC, and qPCR. Exposure decreased visual behavior and the thickness of most retinal layers. Exposure decreased expression of , , , and . Exposure increased expression of , , , , , , , and , and increased retinal brain aromatase expression by IHC. Collectively, our findings demonstrate that nitrate and nitrite exposure negatively impacted the visual system of adult zebrafish, highlighting the potential hazards of these environmental pollutants on aquatic organisms.
硝酸盐和亚硝酸盐已成为日益常见的环境污染物，对生态系统中的各种生命形式构成重大风险。为了解它们对斑马鱼视觉系统的影响，将成年斑马鱼暴露于环境相关浓度的硝酸盐（10毫克/升）和亚硝酸盐（1毫克/升）中7天。使用视动反应和回避反应检查视觉行为。收集斑马鱼的眼球进行苏木精-伊红染色、免疫组织化学和定量聚合酶链反应。暴露降低了视觉行为和大多数视网膜层的厚度。暴露降低了……、……、……和……的表达。暴露增加了……、……、……、……、……、……、……和……的表达，并通过免疫组织化学增加了视网膜脑芳香化酶的表达。总体而言，我们的研究结果表明，硝酸盐和亚硝酸盐暴露对成年斑马鱼的视觉系统产生了负面影响，突出了这些环境污染物对水生生物的潜在危害。 （注：原文中部分基因名称未完整给出，用省略号代替）
