Muscarà Claudia, Speciale Antonio, Molonia Maria Sofia, Salamone Federica Lina, Saija Antonella, Cimino Francesco
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Nat Prod Res. 2024 Jul 26:1-11. doi: 10.1080/14786419.2024.2384080.
Gut epithelial barrier perturbation leads to leaky gut syndrome and permeation of substances activating immune response. Polyphenols can improve intestinal barrier function and represent candidates for preventing development of leaky gut. Herein, we evaluated the molecular mechanisms involved in the protective effects of a polyphenol-rich extract from leaves of L. (CCLE) on intestinal barrier function and integrity on Caco-2 human epithelial cells. Treatment with CCLE from seeding until complete differentiation improved intestinal function by increasing trans-epithelial electrical resistance (TEER), reducing paracellular permeability to fluorescein, and promoting faster recovery of tight junctions (TJ) assembly in the Ca switch assay. CCLE stimulated epithelial cell differentiation inducing alkaline phosphatase activity and TJ proteins. These CCLE-induced effects were attributed to activation of AMP-activated protein kinase (AMPK) pathway. Our data support the use of L. leaves, an agricultural co-product rich in bioactive polyphenols, for the health of intestinal epithelium.
肠道上皮屏障紊乱会导致肠漏综合征以及激活免疫反应的物质渗透。多酚可以改善肠道屏障功能，是预防肠漏发生的潜在物质。在此，我们评估了来自L.（CCLE）叶的富含多酚的提取物对Caco-2人上皮细胞肠道屏障功能和完整性的保护作用所涉及的分子机制。从接种到完全分化期间用CCLE处理，通过增加跨上皮电阻（TEER）、降低荧光素的细胞旁通透性以及在钙切换试验中促进紧密连接（TJ）组装的更快恢复来改善肠道功能。CCLE刺激上皮细胞分化，诱导碱性磷酸酶活性和TJ蛋白。这些CCLE诱导的作用归因于AMP激活的蛋白激酶（AMPK）途径的激活。我们的数据支持将富含生物活性多酚的农业副产品L.叶用于肠道上皮健康。
