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Rapid identification of thermophilic Naegleria, including Naegleria fowleri using API ZYM system.使用API ZYM系统快速鉴定嗜热性耐格里属，包括福氏耐格里阿米巴。
J Clin Pathol. 1985 Nov;38(11):1289-92. doi: 10.1136/jcp.38.11.1289.
2
Identification of Naegleria fowleri in fresh isolates of environmental amoebae using a staphylococcal coagglutination technique.
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The genome of Naegleria lovaniensis, the basis for a comparative approach to unravel pathogenicity factors of the human pathogenic amoeba N. fowleri.嗜热双口虫（Naegleria lovaniensis）基因组，为解析人类致病阿米巴福氏耐格里虫（N. fowleri）致病因子的比较方法提供了基础。
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引用本文的文献

1
Biology of Naegleria spp.耐格里属生物
Microbiol Rev. 1988 Mar;52(1):114-33. doi: 10.1128/mr.52.1.114-133.1988.

本文引用的文献

1
Primary amoebic meningoencephalitis.原发性阿米巴脑膜脑炎
Arch Dis Child. 1981 Feb;56(2):140-3. doi: 10.1136/adc.56.2.140.
2
Determination of acid phosphatase and leucine amino peptidase activity as an identification method for pathogenic Naegleria fowleri.
Trans R Soc Trop Med Hyg. 1982;76(6):773-5. doi: 10.1016/0035-9203(82)90104-3.
3
Hospital hydrotherapy pools treated with ultra violet light: bad bacteriological quality and presence of thermophilic Naegleria.经紫外线处理的医院水疗池：细菌学质量差且存在嗜热性耐格里属阿米巴。
J Hyg (Lond). 1982 Apr;88(2):205-14. doi: 10.1017/s0022172400070078.
4
Naegleria lovaniensis new species: isolation and identification of six thermophilic strains of a new species found in association with Naegleria fowleri.洛万内氏耐格里变形虫新种：与福氏耐格里变形虫相关的一个新种的六个嗜热菌株的分离与鉴定
Int J Parasitol. 1980 Feb;10(1):51-64. doi: 10.1016/0020-7519(80)90064-8.
5
Isolation and identification of pathogenic Naegleria australiensis (De Jonckheere, 1981) from pond water in India.
Trans R Soc Trop Med Hyg. 1983;77(6):878. doi: 10.1016/0035-9203(83)90317-6.
6
Isolation and identification of pathogenic Naegleria australiensis (Amoebida, Vahlkampfiidae) from a spa in northern Italy.从意大利北部一个温泉浴场分离并鉴定致病性澳大利亚福氏耐格里阿米巴（变形虫纲，瓦氏变形虫科）
Appl Environ Microbiol. 1983 Dec;46(6):1282-5. doi: 10.1128/aem.46.6.1282-1285.1983.
7
Differentiation between Naegleria fowleri and N. lovaniensis using isoenzyme electrophoresis of aspartate aminotransferase.
Trans R Soc Trop Med Hyg. 1984;78(4):562-3. doi: 10.1016/0035-9203(84)90088-9.
8
A comparative study of 14 strains of Naegleria australiensis demonstrates the existence of a highly virulent subspecies: N. australiensis italica n. spp.
J Protozool. 1984 May;31(2):324-31. doi: 10.1111/j.1550-7408.1984.tb02971.x.
9
Primary amebic meningoencephalitis.原发性阿米巴脑膜脑炎
N Engl J Med. 1966 Jun 30;274(26):1473-6. doi: 10.1056/NEJM196606302742605.
10
Use of an axenic medium for differentiation between pathogenic and nonpathogenic Naegleria fowleri isolates.使用无菌培养基区分致病和非致病福氏耐格里阿米巴分离株。
Appl Environ Microbiol. 1977 Apr;33(4):751-7. doi: 10.1128/aem.33.4.751-757.1977.

使用API ZYM系统快速鉴定嗜热性耐格里属，包括福氏耐格里阿米巴。

Rapid identification of thermophilic Naegleria, including Naegleria fowleri using API ZYM system.

作者信息

Kilvington S, White D G

出版信息

J Clin Pathol. 1985 Nov;38(11):1289-92. doi: 10.1136/jcp.38.11.1289.

DOI:10.1136/jcp.38.11.1289
PMID:3905871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499429/
Abstract

The suitability of the API ZYM system for identifying thermophilic Naegleria species, based on enzyme presence and activity, was investigated. Replicate testing on strains of N fowleri, N lovaniensis, and N australiensis cultured in a monoxenic and an axenic medium showed that the system could provide a rapid and reproducible means of identifying the species soon after primary isolation. No single enzyme was found specific for any one species, but considerable differences were found in the patterns of activity of acid phosphatase and leucine arylamidase. When these were compared the species could be differentiated. Use of the system in conjunction with a simple culture method is proposed as a readily available means of monitoring environmental and public bathing sites to prevent primary amoebic meningoencephalitis.

摘要

基于酶的存在和活性，研究了API ZYM系统用于鉴定嗜热性耐格里属物种的适用性。在单菌培养和无菌培养基中培养的福氏耐格里阿米巴、洛万尼耐格里阿米巴和澳大利亚耐格里阿米巴菌株上进行的重复测试表明，该系统可以在初次分离后不久提供一种快速且可重复的物种鉴定方法。未发现单一酶对任何一个物种具有特异性，但在酸性磷酸酶和亮氨酸芳基酰胺酶的活性模式上发现了相当大的差异。当对这些进行比较时，可以区分物种。建议将该系统与一种简单的培养方法结合使用，作为一种随时可用的手段来监测环境和公共浴场，以预防原发性阿米巴脑膜脑炎。