使用API ZYM系统快速鉴定嗜热性耐格里属，包括福氏耐格里阿米巴。
Rapid identification of thermophilic Naegleria, including Naegleria fowleri using API ZYM system.
作者信息
Kilvington S, White D G
出版信息
J Clin Pathol. 1985 Nov;38(11):1289-92. doi: 10.1136/jcp.38.11.1289.
Abstract
The suitability of the API ZYM system for identifying thermophilic Naegleria species, based on enzyme presence and activity, was investigated. Replicate testing on strains of N fowleri, N lovaniensis, and N australiensis cultured in a monoxenic and an axenic medium showed that the system could provide a rapid and reproducible means of identifying the species soon after primary isolation. No single enzyme was found specific for any one species, but considerable differences were found in the patterns of activity of acid phosphatase and leucine arylamidase. When these were compared the species could be differentiated. Use of the system in conjunction with a simple culture method is proposed as a readily available means of monitoring environmental and public bathing sites to prevent primary amoebic meningoencephalitis.
摘要
基于酶的存在和活性，研究了API ZYM系统用于鉴定嗜热性耐格里属物种的适用性。在单菌培养和无菌培养基中培养的福氏耐格里阿米巴、洛万尼耐格里阿米巴和澳大利亚耐格里阿米巴菌株上进行的重复测试表明，该系统可以在初次分离后不久提供一种快速且可重复的物种鉴定方法。未发现单一酶对任何一个物种具有特异性，但在酸性磷酸酶和亮氨酸芳基酰胺酶的活性模式上发现了相当大的差异。当对这些进行比较时，可以区分物种。建议将该系统与一种简单的培养方法结合使用，作为一种随时可用的手段来监测环境和公共浴场，以预防原发性阿米巴脑膜脑炎。
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本文引用的文献
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Primary amoebic meningoencephalitis.原发性阿米巴脑膜脑炎
Arch Dis Child. 1981 Feb;56(2):140-3. doi: 10.1136/adc.56.2.140.
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Determination of acid phosphatase and leucine amino peptidase activity as an identification method for pathogenic Naegleria fowleri.
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Hospital hydrotherapy pools treated with ultra violet light: bad bacteriological quality and presence of thermophilic Naegleria.经紫外线处理的医院水疗池：细菌学质量差且存在嗜热性耐格里属阿米巴。
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Isolation and identification of pathogenic Naegleria australiensis (De Jonckheere, 1981) from pond water in India.
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