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猪胴体细菌采样的拭子法与浸渍法比较。

A comparison of swab and maceration methods for bacterial sampling of pig carcasses.

作者信息

Morgan I R, Krautil F, Craven J A

出版信息

J Hyg (Lond). 1985 Oct;95(2):383-90. doi: 10.1017/s002217240006280x.

DOI:10.1017/s002217240006280x
PMID:3905957
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129550/
Abstract

A swabbing technique was compared with an excision and maceration technique for bacteriological sampling of pig carcass skin surfaces. Total viable counts at 37 degrees C obtained by swabbing were 46% of those obtained by maceration. At 21 degrees C, swabbing gave total viable counts which were 54% of the counts obtained from excision samples. Escherichia coli counts showed wide variation with both sampling methods. Neither method was more efficient than the other in recovering E. coli, although excision sampling gave generally higher counts. Both methods were equally effective at recovering salmonellae from carcass surfaces. There was no significant difference between the methods in recovering particular Salmonella serotypes.

摘要

对猪胴体皮肤表面进行细菌学采样时,将擦拭技术与切除和浸软技术进行了比较。通过擦拭在37摄氏度下获得的总活菌数是通过浸软获得的总活菌数的46%。在21摄氏度时,擦拭获得的总活菌数是从切除样本中获得的总活菌数的54%。两种采样方法的大肠杆菌计数差异很大。在回收大肠杆菌方面,两种方法都不比另一种方法更有效,尽管切除采样通常得到的计数更高。两种方法在从胴体表面回收沙门氏菌方面同样有效。在回收特定沙门氏菌血清型方面,两种方法之间没有显著差异。

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本文引用的文献

1
Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.通过使用Rappaport-Vassiliadis增菌肉汤改进从天然污染的肉制品中分离沙门氏菌的方法。
Appl Environ Microbiol. 1981 Oct;42(4):615-8. doi: 10.1128/aem.42.4.615-618.1981.
2
A rapid, inexpensive bacterial count technique using agar droplets.一种使用琼脂液滴的快速、低成本细菌计数技术。
J Appl Bacteriol. 1971 Jun;34(2):435-40. doi: 10.1111/j.1365-2672.1971.tb02303.x.
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A rapid and direct plate method for enumerating Escherichia coli biotype I in food.
J Appl Bacteriol. 1975 Oct;39(2):111-7. doi: 10.1111/j.1365-2672.1975.tb00551.x.
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The microbiology of the red meat carcass and the slaughterhouse.红肉胴体及屠宰场的微生物学
R Soc Health J. 1976 Dec;96(6):270-6.