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通过使用Rappaport-Vassiliadis增菌肉汤改进从天然污染的肉制品中分离沙门氏菌的方法。

Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.

作者信息

Vassiliadis P, Kalapothaki V, Trichopoulos D, Mavrommatti C, Serie C

出版信息

Appl Environ Microbiol. 1981 Oct;42(4):615-8. doi: 10.1128/aem.42.4.615-618.1981.

DOI:10.1128/aem.42.4.615-618.1981
PMID:7337465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC244071/
Abstract

A total of 454 specimens of meat products were examined for salmonellae by using five procedures of enrichment. The use of a selective motility medium, inoculated from enrichment in Muller-Kauffmann broth, resulted in an increase in the number of positive specimens. However, simple enrichment in Rappaport-Vassiliadis broth, after preenrichment, was more sensitive and specific for recovering salmonellae than the selective motility medium-Muller-Kauffmann broth method.

摘要

共采用五种增菌程序对454份肉制品样本进行沙门氏菌检测。使用从Muller-Kauffmann肉汤增菌液接种的选择性动力培养基，可使阳性样本数量增加。然而，预增菌后在Rappaport-Vassiliadis肉汤中进行简单增菌，在沙门氏菌的回收方面比选择性动力培养基-Muller-Kauffmann肉汤法更敏感、更具特异性。

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Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.通过使用Rappaport-Vassiliadis增菌肉汤改进从天然污染的肉制品中分离沙门氏菌的方法。
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2
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引用本文的文献

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Front Microbiol. 2016 Dec 27;7:2103. doi: 10.3389/fmicb.2016.02103. eCollection 2016.
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本文引用的文献

1
A new enrichment medium for certain Salmonellae.一种用于某些沙门氏菌的新型富集培养基。
J Clin Pathol. 1956 Aug;9(3):261-6. doi: 10.1136/jcp.9.3.261.
2
Salmonella isolation with Rappaport's enrichment medium of different compositions.使用不同成分的拉帕波特富集培养基分离沙门氏菌。
Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(5):382-9.
3
Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium.使用100毫升拉帕波特富集培养基的R10改良培养基分离沙门氏菌。
J Hyg (Lond). 1981 Aug;87(1):35-41. doi: 10.1017/s0022172400069217.
4
A method of secondary enrichment for salmonellas independent of selectively toxic chemicals.一种不依赖选择性有毒化学物质的沙门氏菌二次富集方法。
J Hyg (Lond). 1966 Sep;64(3):361-6. doi: 10.1017/s002217240004064x.
5
A selective motility medium for routine isolation of Salmonella.一种用于常规分离沙门氏菌的选择性动力培养基。
J Hyg (Lond). 1969 Jun;67(2):181-6. doi: 10.1017/s0022172400041589.
6
[A new procedure of "Salmonella" enrichment (author's transl)].["沙门氏菌"增菌新方法（作者译）]
Ann Microbiol (Paris). 1976 Aug-Sep;127B(2):195-200.
7
Enrichment procedures for isolating salmonellae from raw meat and poultry.从生肉和家禽中分离沙门氏菌的富集程序。
Appl Environ Microbiol. 1978 Oct;36(4):627-8. doi: 10.1128/aem.36.4.627-628.1978.
8
Brilliant green deoxycholate agar as an improved selective medium for the isolation of Salmonella.
Ann Soc Belg Med Trop. 1979 Jun;59(2):117-20.
9
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique.使用预富集技术比较亚硒酸盐F、穆勒-考夫曼四硫磺酸盐和拉帕波特培养基从受污水污染的天然水中分离沙门氏菌的效果。
J Hyg (Lond). 1979 Dec;83(3):451-60. doi: 10.1017/s0022172400026292.
10
Secondary selective enrichment of salmonellae from naturally contaminated specimens by using a selective motility system.通过使用选择性运动系统从自然污染标本中对沙门氏菌进行二次选择性富集。
Appl Environ Microbiol. 1979 Apr;37(4):725-8. doi: 10.1128/aem.37.4.725-728.1979.