通过使用Rappaport-Vassiliadis增菌肉汤改进从天然污染的肉制品中分离沙门氏菌的方法。
Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.
作者信息
Vassiliadis P, Kalapothaki V, Trichopoulos D, Mavrommatti C, Serie C
出版信息
Appl Environ Microbiol. 1981 Oct;42(4):615-8. doi: 10.1128/aem.42.4.615-618.1981.
Abstract
A total of 454 specimens of meat products were examined for salmonellae by using five procedures of enrichment. The use of a selective motility medium, inoculated from enrichment in Muller-Kauffmann broth, resulted in an increase in the number of positive specimens. However, simple enrichment in Rappaport-Vassiliadis broth, after preenrichment, was more sensitive and specific for recovering salmonellae than the selective motility medium-Muller-Kauffmann broth method.
摘要
共采用五种增菌程序对454份肉制品样本进行沙门氏菌检测。使用从Muller-Kauffmann肉汤增菌液接种的选择性动力培养基，可使阳性样本数量增加。然而，预增菌后在Rappaport-Vassiliadis肉汤中进行简单增菌，在沙门氏菌的回收方面比选择性动力培养基-Muller-Kauffmann肉汤法更敏感、更具特异性。
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本文引用的文献
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Salmonella isolation with Rappaport's enrichment medium of different compositions.使用不同成分的拉帕波特富集培养基分离沙门氏菌。
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Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium.使用100毫升拉帕波特富集培养基的R10改良培养基分离沙门氏菌。
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Secondary selective enrichment of salmonellae from naturally contaminated specimens by using a selective motility system.通过使用选择性运动系统从自然污染标本中对沙门氏菌进行二次选择性富集。
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