糖尿病患者抗胰岛素抗体的固相酶免疫测定法。
A solid-phase enzyme immunoassay for anti-insulin antibody in diabetes mellitus patients.
作者信息
Kobayashi N, Terada E, Ghoda A, Kitagaki K, Okudaira H, Ogita T, Shimabukuro K, Miyamoto T
出版信息
J Immunol Methods. 1985 Nov 28;84(1-2):245-50. doi: 10.1016/0022-1759(85)90431-4.
A solid-phase enzyme immunoassay for the measurement of anti-insulin antibodies in the sera of patients with diabetes mellitus was developed. Porcine insulin conjugated with bovine serum albumin was used for coating microtiter plates. This assay was as sensitive as the conventional radioimmunoassay. Anti-insulin antibody titers measured by the enzyme immunoassay and conventional radioimmunoassay correlated well and the correlation coefficient was 0.920, which was statistically significant (P less than 0.001). The enzyme immunoassay detected anti-insulin antibody in 23 out of 35 sera of patients with insulin-dependent diabetes mellitus. The present enzyme immunoassay does not require radioactive materials, is less expensive and is concluded to be practically useful.
开发了一种用于测定糖尿病患者血清中抗胰岛素抗体的固相酶免疫测定法。将与牛血清白蛋白偶联的猪胰岛素用于包被微量滴定板。该测定法与传统放射免疫测定法一样灵敏。通过酶免疫测定法和传统放射免疫测定法测得的抗胰岛素抗体滴度相关性良好，相关系数为0.920，具有统计学意义（P小于0.001）。酶免疫测定法在35例胰岛素依赖型糖尿病患者的血清中检测出23例含有抗胰岛素抗体。目前的酶免疫测定法不需要放射性物质，成本较低，因此被认为具有实际应用价值。
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