Department of Oral and Maxillofacial Surgery, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Oral and Maxillofacial Surgery, Tabriz University of Medical Sciences, Tabriz, Iran.
J Oral Biosci. 2024 Dec;66(4):107-115. doi: 10.1016/j.job.2024.07.007. Epub 2024 Jul 24.
To examine the potential of intra-articular administration of mesenchymal stem cells (MSCs) derived from bone marrow or adipose tissue to mitigate synovial inflammation in a rat model of temporomandibular joint (TMJ) osteoarthritis (OA).
In this experimental study, 40 rats were divided into 4 groups: (1) Control group; (2) Untreated TMJ-OA group; (3) TMJ-OA group treated with bone marrow-derived MSCs; (4) TMJ-OA group treated with adipose tissue-derived MSCs. The TMJ-OA model was established by inducing synovial inflammation through the intra-articular administration of complete Freund's adjuvant (CFA). After 8 weeks of TMJ-OA establishment, the animals were sacrificed and each mandibular condyle was extracted for histological evaluation.
The untreated TMJ-OA group had significantly higher synovial inflammation, as indicated microscopically by higher grades of synovial membrane hyperplasia and adhesion, vascular vasodilation, and fibrin deposition than the control group (p < 0.001). Both TMJ-OA groups treated with MSCs had lower grades of synovial inflammation and less severe synovitis than the untreated TMJ-OA group (p < 0.001). The TMJ-OA group treated with adipose tissue-derived MSCs showed lower grades of synovial membrane hyperplasia and higher grades of fibrin deposition than the that treated with bone marrow-derived MSCs (p < 0.001). Other indicators of synovial inflammation and synovitis severity were comparable between the two treatment groups.
Administration of CFA to the TMJ-OA rat model augmented synovial inflammation. Intra-articular administration of MSCs derived from either bone marrow or adipose tissue attenuated the microscopic manifestations of this inflammation, indicating the therapeutic potential of this treatment for TMJ-OA.
研究骨髓间充质干细胞（MSCs）和脂肪来源间充质干细胞（ADSCs）关节内注射对大鼠颞下颌关节（TMJ）骨关节炎（OA）滑膜炎症的缓解作用。
本实验研究将 40 只大鼠分为 4 组：（1）对照组；（2）未治疗 TMJ-OA 组；（3）骨髓来源 MSC 治疗的 TMJ-OA 组；（4）脂肪来源 MSC 治疗的 TMJ-OA 组。通过关节内注射完全弗氏佐剂（CFA）诱导滑膜炎症，建立 TMJ-OA 模型。建立 TMJ-OA 模型 8 周后，处死动物并取出每个下颌骨髁进行组织学评估。
未治疗的 TMJ-OA 组滑膜炎症明显高于对照组，滑膜膜增生和粘连、血管扩张和纤维蛋白沉积的分级较高（p<0.001）。与未治疗的 TMJ-OA 组相比，MSC 治疗的两组 TMJ-OA 组的滑膜炎症分级较低，滑膜炎较轻（p<0.001）。与骨髓来源 MSC 治疗的组相比，脂肪来源 MSC 治疗的组滑膜膜增生分级较低，纤维蛋白沉积分级较高（p<0.001）。两组间滑膜炎症和滑膜炎严重程度的其他指标无差异。
CFA 注射到 TMJ-OA 大鼠模型中可增强滑膜炎症。关节内注射骨髓或脂肪来源的 MSC 可减轻这种炎症的微观表现，表明这种治疗方法对 TMJ-OA 具有治疗潜力。
