School of Grain Science and Technology, Jiangsu University of Science and Technology, 212100 Zhenjiang, China.
Food and Bioproducts, Production Systems Unit, Natural Resources Institute Finland (Luke), FI-31600 Jokioinen, Finland.
Food Res Int. 2024 Sep;191:114696. doi: 10.1016/j.foodres.2024.114696. Epub 2024 Jun 27.
Baltic herring is the main catch in the Baltic Sea; however, its usage could be improved due to the low processing rate. Previously we have shown that whole Baltic herring hydrolysates (BHH) and herring byproducts hydrolysates (BHBH) by commercial enzymes consisted of bioactive peptides and had moderate bioactivity in in vitro dipeptidyl peptidase (DPP)-4 assay. In this study, we identified the hydrolysate peptides by LC-MS/MS and predicted the potential bioactive DPP-4 inhibitory peptides using in silico tools. Based on abundance, peptide length and stability, 86 peptides from BHBH and 80 peptides from BHH were proposed to be novel DPP-4 inhibitory peptides. BHH was fed to a mice intervention of a high-fat, high-fructose diet to validate the bioactivity. The results of the glucose tolerance and insulin tolerance improved. Plasma DPP-4 activities, C-peptide levels, and HOMA-IR scores significantly decreased, while plasma glucagon-like peptide-1 content increased. In conclusion, BHH is an inexpensive and sustainable source of functional antidiabetic ingredients.
波罗的海鲱鱼是波罗的海中的主要捕捞物，但由于加工率低，其用途可以得到改善。此前我们已经表明，全波罗的海鲱鱼水解物（BHH）和鲱鱼副产物水解物（BHBH）由商业酶组成，含有生物活性肽，在体外二肽基肽酶（DPP-4）测定中具有中等的生物活性。在这项研究中，我们通过 LC-MS/MS 鉴定了水解物肽，并使用计算机工具预测了潜在的生物活性 DPP-4 抑制肽。基于丰度、肽长度和稳定性，从 BHBH 中提出了 86 个肽，从 BHH 中提出了 80 个肽，它们可能是新的 DPP-4 抑制肽。BHH 被喂食给高脂肪、高果糖饮食的小鼠干预，以验证其生物活性。葡萄糖耐量和胰岛素耐量的结果得到改善。血浆 DPP-4 活性、C 肽水平和 HOMA-IR 评分显著降低，而胰高血糖素样肽-1 含量增加。总之，BHH 是一种廉价且可持续的功能性抗糖尿病成分来源。
