Zhang Zhenxing, Chen Xiangying, Meng Yong, Jiang Junming, Wu Lili, Chen Taoyu, Pan Haoju, Jiao Zizhuo, Du Li, Man Churiga, Chen Si, Wang Fengyang, Gao Hongyan, Chen Qiaoling
Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Animals (Basel). 2024 Jul 14;14(14):2064. doi: 10.3390/ani14142064.
subsp. (Mcc), a member of the cluster, has a negative impact on the goat-breeding industry. However, little is known about the pathogenic mechanism of Mcc. This study infected mice using a previously isolated strain, Mcc HN-B. Hematoxylin and eosin staining, RNA sequencing, bioinformatic analyses, RT-qPCR, and immunohistochemistry were performed on mouse lung tissues. The results showed that 235 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses suggested that the DEGs were mainly associated with immune response, defensive response to bacteria, NF-kappa B signaling pathway, natural killer cell-mediated cytotoxicity, and T cell receptor signaling pathway. RT-qPCR verified the expression of , , , , , , , , , and . The up-regulation of S100A8 and S100A9 at the protein level was confirmed by immunohistochemistry. Moreover, RT-qPCR assays on Mcc HN-B-infected RAW264.7 cells also showed that the expression of and was elevated. S100A8 and S100A9 not only have diagnostic value in Mcc infection but also hold great significance in clarifying the pathogenic mechanism of Mcc. This study preliminarily elucidates the mechanism of Mcc HN-B-induced lung injury and provides a theoretical basis for further research on Mcc-host interactions.
亚种（Mcc）是该菌群的一个成员，对山羊养殖业有负面影响。然而，关于Mcc的致病机制知之甚少。本研究使用先前分离的菌株Mcc HN - B感染小鼠。对小鼠肺组织进行苏木精和伊红染色、RNA测序、生物信息学分析、RT - qPCR和免疫组织化学。结果表明，共鉴定出235个差异表达基因（DEGs）。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，这些DEGs主要与免疫反应、对细菌的防御反应、核因子κB信号通路、自然杀伤细胞介导的细胞毒性以及T细胞受体信号通路有关。RT - qPCR验证了[具体基因名称未给出]等基因的表达。免疫组织化学证实了S100A8和S100A9在蛋白质水平上的上调。此外，对Mcc HN - B感染的RAW264.7细胞进行的RT - qPCR分析也表明[具体基因名称未给出]的表达升高。S100A8和S100A9不仅在Mcc感染中具有诊断价值，而且在阐明Mcc的致病机制方面也具有重要意义。本研究初步阐明了Mcc HN - B诱导肺损伤的机制，为进一步研究Mcc与宿主相互作用提供了理论依据。
