Abdelgawad Raghda Magdy, Damé-Teixeira Nailê, Gurzawska-Comis Katarzyna, Alghamdi Arwa, Mahran Abeer H, Elbackly Rania, Do Thuy, El-Gendy Reem
Division of Oral Biology, Leeds School of Dentistry, St. James University Hospital, University of Leeds, Leeds LS9 7TF, UK.
Department of Endodontics, Faculty of Dentistry, Assiut University, Assiut 83523, Egypt.
Bioengineering (Basel). 2024 Jun 26;11(7):653. doi: 10.3390/bioengineering11070653.
Regenerative endodontics (REP) is a new clinical modality aiming to regenerate damaged soft and hard dental tissues, allowing for root completion in young adults' teeth. Effective disinfection is crucial for REP success, but commonly used antimicrobials often harm the niche dental pulp stem cells (DPSCs). To our knowledge, this is the first study to explore the biocompatibility and antimicrobial potential of pectin as a potential natural intracanal medicament for REPs. Low methoxyl commercial citrus pectin (LM) (pectin CU701, Herbstreith&Fox.de) was used in all experiments. The pectin's antibacterial activity against single species biofilms ( and was assessed using growth curves. The pectin's antimicrobial effect against mature dual-species biofilm was also evaluated using confocal laser scanning microscopy (CLSM) after 30 min and 7 days of treatment. The DPSC biocompatibility with 2% and 4% / of the pectin coatings was evaluated using live/dead staining, LDH, and WST-1 assays. Pectin showed a concentration-dependent inhibitory effect against single-species biofilms ( and but failed to disrupt dual-species biofilm. Pectin at 2% / concentration proved to be biocompatible with the HDPSCs. However, 4% / pectin reduced both the viability and proliferation of the DPSCs. Low concentration (2% /) pectin was biocompatible with the DPSCs and showed an antimicrobial effect against single-species biofilms. This suggests the potential for using pectin as an injectable hydrogel for clinical applications in regenerative endodontics.
再生牙髓治疗（REP）是一种新的临床治疗方式，旨在使受损的牙齿软硬组织再生，实现年轻恒牙的牙根发育完成。有效的消毒对于REP的成功至关重要，但常用的抗菌剂往往会损害牙髓干细胞（DPSCs）微环境。据我们所知，这是第一项探索果胶作为REP潜在的天然根管内药物的生物相容性和抗菌潜力的研究。所有实验均使用低甲氧基商业柑橘果胶（LM）（果胶CU701，Herbstreith&Fox.de）。通过生长曲线评估果胶对单一菌种生物膜（和）的抗菌活性。在治疗30分钟和7天后，还使用共聚焦激光扫描显微镜（CLSM）评估果胶对成熟双菌种生物膜的抗菌效果。使用活/死染色、乳酸脱氢酶（LDH）和WST-1检测评估2%和4%/果胶涂层与DPSC的生物相容性。果胶对单一菌种生物膜（和）显示出浓度依赖性抑制作用，但未能破坏双菌种生物膜。2%/浓度的果胶被证明与人牙髓干细胞（HDPSCs）具有生物相容性。然而，4%/的果胶降低了DPSCs的活力和增殖。低浓度（2%/）果胶与DPSCs具有生物相容性，并对单一菌种生物膜显示出抗菌作用。这表明果胶作为可注射水凝胶在再生牙髓治疗临床应用中的潜力。
