Király József, Szabó Erzsébet, Fodor Petra, Vass Anna, Choudhury Mahua, Gesztelyi Rudolf, Szász Csaba, Flaskó Tibor, Dobos Nikoletta, Zsebik Barbara, Steli Ákos József, Halmos Gábor, Szabó Zsuzsanna
Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, Hungary.
Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032 Debrecen, Hungary.
Biomedicines. 2024 Jun 27;12(7):1441. doi: 10.3390/biomedicines12071441.
MicroRNAs (miRNAs) play a regulatory role in various human cancers. The roles of hsa-miR-15a-5p, hsa-miR-99b-5p, and hsa-miR-181a-5p have not been fully explored in the angiogenesis of renal cell carcinoma (RCC).
The present study aimed to evaluate the expression of these miRNAs in tumorous and adjacent healthy tissues of RCC.
Paired tumorous and adjacent normal kidney tissues from 20 patients were studied. The expression levels of hsa-miR-15b-5p, hsa-miR-99b-5p, and hsa-miR-181a-5p were quantified by TaqMan miRNA Assays. Putative targets were analyzed by qRT-PCR.
Significant downregulation of all three miRNAs investigated was observed in tumorous samples compared to adjacent normal kidney tissues. Spearman analysis showed a negative correlation between the expression levels of miRNAs and the pathological grades of the patients. Increased expression of vascular endothelial growth factor-A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α), a tissue inhibitor of metalloproteinases-1 (TIMP-1), was observed in tumorous samples compared to adjacent normal tissues. Depletion of tissue inhibitors of metalloproteinase-2 (TIMP-2) and metalloproteinase-2 (MMP-2) was detected compared to normal adjacent tissues. The examined miRNAs might function as contributing factors to renal carcinogenesis. However, more prospective studies are warranted to evaluate the potential role of miRNAs in RCC angiogenesis.
微小RNA（miRNA）在多种人类癌症中发挥调节作用。hsa-miR-15a-5p、hsa-miR-99b-5p和hsa-miR-181a-5p在肾细胞癌（RCC）血管生成中的作用尚未得到充分研究。
本研究旨在评估这些miRNA在RCC肿瘤组织和相邻健康组织中的表达。
研究了20例患者的配对肿瘤组织和相邻正常肾组织。通过TaqMan miRNA检测法定量hsa-miR-15b-5p、hsa-miR-99b-5p和hsa-miR-181a-5p的表达水平。通过qRT-PCR分析潜在靶点。
与相邻正常肾组织相比，在肿瘤样本中观察到所有三种研究的miRNA均显著下调。Spearman分析显示miRNA表达水平与患者病理分级之间呈负相关。与相邻正常组织相比，在肿瘤样本中观察到血管内皮生长因子-A（VEGF-A）和缺氧诱导因子-1α（HIF-1α）、金属蛋白酶组织抑制剂-1（TIMP-1）的表达增加。与相邻正常组织相比，检测到金属蛋白酶组织抑制剂-2（TIMP-2）和金属蛋白酶-2（MMP-2）的消耗。所检测的miRNA可能是肾癌发生的促成因素。然而，需要更多的前瞻性研究来评估miRNA在RCC血管生成中的潜在作用。
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