博茨瓦纳农村和城郊社区感染艾滋病毒人群中乙型肝炎病毒的分子特征分析

Molecular Characterization of Hepatitis B Virus in People Living with HIV in Rural and Peri-Urban Communities in Botswana.

作者信息

Phinius Bonolo B, Choga Wonderful T, Anderson Motswedi, Mokomane Margaret, Gobe Irene, Ratsoma Tsholofelo, Phakedi Basetsana, Mpebe Gorata, Bhebhe Lynnette, Gaolathe Tendani, Mosepele Mosepele, Makhema Joseph, Shapiro Roger, Lockman Shahin, Musonda Rosemary, Moyo Sikhulile, Gaseitsiwe Simani

机构信息

Botswana Harvard Health Partnership, Gaborone Private Bag BO320, Botswana.

School of Allied Health Professions, Faculty of Health Sciences, University of Botswana, Gaborone Private Bag UB0022, Botswana.

出版信息

Biomedicines. 2024 Jul 14;12(7):1561. doi: 10.3390/biomedicines12071561.

DOI:10.3390/biomedicines12071561

PMID:39062134

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11275055/

Abstract

(1) Background: Hepatitis B virus (HBV) sequencing data are important for monitoring HBV evolution. We aimed to molecularly characterize HBV sequences from participants with HBV surface antigen-positive (HBsAg+) serology and occult hepatitis B infection (OBI+). (2) Methods: We utilized archived plasma samples from people living with human immunodeficiency virus (PLWH) in Botswana. HBV DNA was sequenced, genotyped and analyzed for mutations. We compared mutations from study sequences to those from previously generated HBV sequences in Botswana. The impact of OBI-associated mutations on protein function was assessed using the Protein Variation Effect Analyzer. (3) Results: Sequencing success was higher in HBsAg+ than in OBI+ samples [86/128 (67.2%) vs. 21/71 (29.2%)]. Overall, 93.5% (100/107) of sequences were genotype A1, 2.8% (3/107) were D3 and 3.7% (4/107) were E. We identified 13 escape mutations in 18/90 (20%) sequences with HBsAg coverage, with K122R having the highest frequency. The mutational profile of current sequences differed from previous Botswana HBV sequences, suggesting possible mutational changes over time. Mutations deemed to have an impact on protein function were Q6H, V194A and W28L. (4) Conclusions: We characterized HBV sequences from PLWH in Botswana. Escape mutations were prevalent and were not associated with OBI. Longitudinal HBV studies are needed to investigate HBV natural evolution.

摘要

(1) 背景：乙型肝炎病毒（HBV）测序数据对于监测HBV的进化很重要。我们旨在对HBV表面抗原阳性（HBsAg+）血清学和隐匿性乙型肝炎感染（OBI+）参与者的HBV序列进行分子特征分析。(2) 方法：我们利用了博茨瓦纳人类免疫缺陷病毒感染者（PLWH）的存档血浆样本。对HBV DNA进行测序、基因分型并分析突变情况。我们将研究序列中的突变与博茨瓦纳先前生成的HBV序列中的突变进行了比较。使用蛋白质变异效应分析仪评估了与OBI相关的突变对蛋白质功能的影响。(3) 结果：HBsAg+样本的测序成功率高于OBI+样本[86/128（67.2%）对21/71（29.2%）]。总体而言，93.5%（100/107）的序列为A1基因型，2.8%（3/107）为D3基因型，3.7%（4/107）为E基因型。我们在18/90（20%）具有HBsAg覆盖的序列中鉴定出13个逃逸突变，其中K122R的频率最高。当前序列的突变谱与博茨瓦纳先前的HBV序列不同，表明可能随时间发生了突变变化。被认为对蛋白质功能有影响的突变是Q6H、V194A和W28L。(4) 结论：我们对博茨瓦纳PLWH的HBV序列进行了特征分析。逃逸突变很普遍，且与OBI无关。需要进行HBV纵向研究以调查HBV的自然进化。